Gallic Acid Is an Active Component for the Anticarcinogenic Action of Grape Seed Procyanidins in Pancreatic Cancer Cells

被引:36
作者
Cedo, Lidia [1 ]
Castell-Auvi, Anna [1 ]
Pallares, Victor [1 ]
Macia, Alba [2 ]
Blay, Mayte [1 ]
Ardevol, Anna [1 ]
Motilva, Maria-Jose [2 ]
Pinent, Montserrat [1 ]
机构
[1] Univ Rovira & Virgili, Dept Bioquim & Biotecnol, Nutrigen Res Grp, E-43007 Tarragona, Spain
[2] Univ Lleida, Escola Tecn Super Engn Agr, Dept Tecnol Dels Aliments, Antioxidants Res Grp, Lleida, Spain
来源
NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL | 2014年 / 66卷 / 01期
关键词
PROANTHOCYANIDINS INDUCE APOPTOSIS; IN-VITRO; INSULIN PRODUCTION; FLAVONOID INTAKE; CARCINOMA-CELLS; BETA-CELLS; EXTRACT; RATS; DEATH; BIOAVAILABILITY;
D O I
10.1080/01635581.2014.851714
中图分类号
R73 [肿瘤学];
学科分类号
100214 [肿瘤学];
摘要
The aim of the present work was to evaluate the effects of a grape seed procyanidin extract (GSPE) on proliferation and apoptosis in the pancreatic adenocarcinoma cell line MIA PaCa-2 and identify the components of the extract with higher activity. The effects of the extract were analyzed on the proliferation and apoptosis processes in MIA PaCa-2 cells, as well as in the levels of the apoptosis markers Bcl-2 and Bax, the mitochondrial membrane potential, and reactive oxygen species levels. Finally, the components of the extract with higher effects were elucidated using enriched fractions of the extract and pure compounds. The results showed that GSPE inhibits cell proliferation and increases apoptosis in MIA PaCa-2 cells, which is primarily mediated by the downregulation of the antiapoptotic protein Bcl-2 and the depolarization of the mitochondrial membrane. GSPE also reduced the formation of reactive oxygen species. The component of the extract that possesses the highest antiproliferative and proapoptotic activity was gallic acid. In conclusion, GSPE acts as anticarcinogenic in MIA PaCa-2 cells, with gallic acid as the major single active constituent of the extract.
引用
收藏
页码:88 / 96
页数:9
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