Diversity of complement factor B/C2 in the common carp (Cyprinus carpio):: three isotypes of B/C2-A expressed in different tissues

被引：40

作者：

Nakao, M ^{[1
]}

Matsumoto, M

Nakazawa, M

Fujiki, K

Yano, T

机构：

[1] Kyushu Univ, Grad Sch Bioresource & Bioenvironm Sci, Lab Marine Biol Chem, Fukuoka 8128581, Japan

[2] Univ Waterloo, Dept Biol, Waterloo, ON N2L 3G1, Canada

来源：

DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY | 2002年 / 26卷 / 06期

关键词：

complement factor B; complement factor C2; gene duplication; diversity; expression; Cyprinus carpio;

D O I：

10.1016/S0145-305X(01)00083-0

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Complement factor B and C2 are two critical proteases for complement activation. Some bony fish have been reported to possess duplicated genes for B/C2, but there is no direct evidence regarding possible functional divergence. Here, we report the isolation of the second and third isotypes of carp B/C2-A. a close relative of other bony fish B reported to date, and designated B/C2-A2 and B/C2-A3. B/C2-A1 (previously reported B/C2-A) and B/C2-A2 share 78% amino acid identity and are synthesized mainly in hepatopancreas. On the other hand. B/C2-A3 showed less (similar to60%) sequence identity with the other two isotypes. It was expressed mainly in kidney and spleen, and was up-regulated after injection of carp with scleroglucan or sodium alginate, known immunostimulants for fish. Phylogenetic analysis suggests that B/C2-A3 diverged before separation of carp and zebrafish. B/C2-A3 represent a novel B/C2-lineage functioning as an acute phase reactant in cyprinid fish. (C) 2002 Published by Elsevier Science Ltd.

引用

页码：533 / 541

页数：9

共 24 条

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