MH2+• ion production from protonated polypeptides by electron impact:: observation and determination of ionization energies and a cross-section

被引：46

作者：

Budnik, BA ^{[1
]}

Zubarev, RA ^{[1
]}

机构：

[1] Univ So Denmark Odense, Dept Chem, DK-5230 Odense M, Denmark

来源：

CHEMICAL PHYSICS LETTERS | 2000年 / 316卷 / 1-2期

关键词：

D O I：

10.1016/S0009-2614(99)01256-7

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Irradiation of gas-phase MH+ ions of polypeptides up to MW 3493 by 11 to 70 eV electrons produced further ionization: MH++ e(-) --> MH2+. + 2 e(-), with ionization thresholds of 11.4 +/- 0.5 eV for [Arg-8]-vasopressin (MW 1084), 10.7 +/- 0.5 eV for substance P (MW 1347), 11.4 +/- 0.6 eV for renin substrate (MW 1759) and 10.6 +/- 0.4 eV for melittin (MW 2846). The ionization cross-section of vasopressin MH+ ions was found to be (1.3 +/- 0.4).10(-15) cm(2) for 20 eV electrons. The gas-phase MH2+. ions are rather stable at room temperature, with lifetimes of hundreds of seconds at 10(-10) Torr. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：19 / 23

页数：5

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