Facilitation of L-type Ca2+ channels in dendritic spines by activation of β2 adrenergic receptors

被引:81
作者
Hoogland, TM [1 ]
Saggau, P [1 ]
机构
[1] Baylor Coll Med, Dept Neurosci, Houston, TX 77030 USA
关键词
dendritic spine; CA1; calcium; L-type; adrenergic; hippocampus; imaging;
D O I
10.1523/JNEUROSCI.1677-04.2004
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
We studied the contribution of L-type Ca2+ channels to action potential-evoked Ca2+ influx in dendritic spines of CA1 pyramidal neurons and the modulation of these channels by the beta(2) adrenergic receptor. Backpropagating action potentials (bAPs) (three at 50 Hz) were evoked by brief somatic current injections, and Ca2+ transients were recorded in proximal basal dendrites and associated spines. The R- and T-type Ca2+ channel blocker NiCl2 (100 muM) significantly reduced Ca2+ transients in both spines and their parent dendrites (similar to50%), suggesting that these channels are the major source of bAP-evoked Ca2+ influx in these structures. The L-type Ca2+ channel blockers nimodipine and nifedipine (both 10 muM) reduced spine Ca2+ transients by similar to10%, whereas the L-type Ca2+ channel activators FPL 64176 (2,5-dimethyl-4-[2-(phenylmethyl)benzoyl]-1H-pyrrole-3-carboxylic acid methylester) and Bay K8644 ((+/-)-1,4-dihydro-2,6-dimethyl-5-nitro-4-[2-(trifluoromethyl)-phenyl]-3-pyridine carboxylic acid methyl ester) (both 10 muM) significantly enhanced the spine Ca2+ transients by 40-50%. Activation of beta(2) adrenergic receptors with salbutamol (40 muM) or formoterol (5 muM) resulted in significant enhancements of the spine (40-50%) but not dendritic Ca2+ transients. This increase was prevented when L-type Ca2+ channels were blocked with nimodipine (10 muM) or when cAMP-dependent protein kinase A (PKA) was inhibited with KT5720 (3 muM), Rp-cAMPS (Rp-adenosine cyclic 3',5'-phosphorothioate) (100 muM), or PKI (100 muM). The above data suggest that L-type Ca2+ channels are functionally present in dendritic spines of CA1 pyramidal neurons, contribute to spine Ca2+ influx, and can be modulated by the beta(2) adrenergic receptor through PKA in a highly compartmentalized manner.
引用
收藏
页码:8416 / 8427
页数:12
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