Determining the specific microbial populations and their spatial distribution within the stromatolite ecosystem of Shark Bay

被引:101
作者
Goh, Falicia [1 ]
Allen, Michelle A. [1 ]
Leuko, Stefan [2 ]
Kawaguchi, Tomohiro [3 ]
Decho, Alan W. [3 ]
Burns, Brendan P. [1 ,2 ]
Neilan, Brett A. [1 ,2 ]
机构
[1] Univ New S Wales, Sch Biotechnol & Biomol Sci, Sydney, NSW 2052, Australia
[2] Univ New S Wales, Australian Ctr Astrobiol, Sydney, NSW 2052, Australia
[3] Univ S Carolina, Arnold Sch Publ Hlth, Dept Environm Hlth Sci, Columbia, SC 29208 USA
基金
美国国家科学基金会; 澳大利亚研究理事会;
关键词
stromatolite; cyanobacteria; FISH; microbial community; geomorphology; phylotype; halophilic archaea; MODERN MARINE STROMATOLITES; LITHIFIED MICRITIC LAMINAE; WESTERN-AUSTRALIA; OXYGEN-CONSUMPTION; SULFATE REDUCTION; SALINITY GRADIENT; HAMELIN POOL; DIVERSITY; CYANOBACTERIA; COMMUNITIES;
D O I
10.1038/ismej.2008.114
中图分类号
Q14 [生态学(生物生态学)];
学科分类号
071012 ; 0713 ;
摘要
The stromatolites at Shark Bay, Western Australia, are analogues of some of the oldest evidence of life on Earth. The aim of this study was to identify and spatially characterize the specific microbial communities associated with Shark Bay intertidal columnar stromatolites. Conventional culturing methods and construction of 16S rDNA clone libraries from community genomic DNA with both universal and specific PCR primers were employed. The estimated coverage, richness and diversity of stromatolite microbial populations were compared with earlier studies on these ecosystems. The estimated coverage for all clone libraries indicated that population coverage was comprehensive. Phylogenetic analyses of stromatolite and surrounding seawater sequences were performed in ARB with the Greengenes database of full-length non-chimaeric 16S rRNA genes. The communities identified exhibited extensive diversity. The most abundant sequences from the stromatolites were alpha- and gamma-proteobacteria (58%), whereas the cyanobacterial community was characterized by sequences related to the genera Euhalothece, Gloeocapsa, Gloeothece, Chroococcidiopsis, Dermocarpella, Acaryochloris, Geitlerinema and Schizothrix. All clones from the archaeal-specific clone libraries were related to the halophilic archaea; however, no archaeal sequence was identified from the surrounding seawater. Fluorescence in situ hybridization also revealed stromatolite surfaces to be dominated by unicellular cyanobacteria, in contrast to the sub-surface archaea and sulphate-reducing bacteria. This study is the first to compare the microbial composition of morphologically similar stromatolites over time and examine the spatial distribution of specific microorganismic groups in these intertidal structures and the surrounding seawater at Shark Bay. The results provide a platform for identifying the key microbial physiology groups and their potential roles in modern stromatolite morphogenesis and ecology.
引用
收藏
页码:383 / 396
页数:14
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