Association of human transferrin receptor with GABARAP

被引：48

作者：

Green, F ^{[1
]}

O'Hare, T ^{[1
]}

Blackwell, A ^{[1
]}

Enns, CA ^{[1
]}

机构：

[1] Oregon Hlth Sci Univ, Dept Cell & Dev Biol, Portland, OR 97201 USA

来源：

FEBS LETTERS | 2002年 / 518卷 / 1-3期

关键词：

transferrin receptor; gamma-aminobutyric acid type A receptor-associated protein; protein trafficking; endocytic motif;

D O I：

10.1016/S0014-5793(02)02655-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A yeast two-hybrid screen identified a specific interaction between the cytoplasmic domain of transferrin receptor (TfR) and GABARAP, a 14 kDa protein that binds to the gamma2 subunit of neuronal GABA(A) receptors. The specificity of the TfR-GABARAP interaction was demonstrated by in vitro binding assays with purified proteins and by co-immunoprecipitation of GABARAP with endogenous TfR from HeLa cell lysates. Replacement of the YTRF internalization motif with ATRA within the cytoplasmic domain of TfR reduced interaction with GABARAP in the yeast two-hybrid screen and in vitro binding assays. The intracellular location of GABARAP using chimeric GABARAP-GFP showed that the majority of GABARAP was located in perinuclear vesicles. Our results show that GABARAP plays a more general role outside the confines of neuronal cells and GABAA receptors. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：101 / 106

页数：6

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