Calorimetric investigation of the adsorption of nitrogen bases and nucleosides on a hydrophobic interaction sorbent

被引：5

作者：

Phillips, JM ^{[1
]}

Pinto, NG ^{[1
]}

机构：

[1] Univ Cincinnati, Dept Chem & Mat Engn, Cincinnati, OH 45221 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 2004年 / 1036卷 / 01期

关键词：

heat of adsorption; hydrophobic interaction chromatography; thermodynamic parameters; oligonucleotides;

D O I：

10.1016/j.chroma.2003.10.129

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Heats of adsorption for nitrogen bases and nucleosides on Sepharose CL-6B, a hydrophobic interaction adsorbent, were collected through flow microcalorimetry in order to ascertain the thermodynamic driving force for adsorption in each case. It was determined that enthalpy changes associated with base stacking self-interactions can contribute significantly to the observed heats of adsorption. Accordingly, the observed heats were the net effect of the adsorbate/adsorbent interactions and the adsorbate stacking self-interactions. Since base stacking proceeds beyond the dimer stage, multi-layer adsorption of these compounds is possible, even at low solution concentrations. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：79 / 86

页数：8

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