Paired helical filaments contain small amounts of cholesterol, phosphatidylcholine and sphingolipids

被引：77

作者：

Gellermann, Gerald P.

Appel, Thomas R.

Davies, Peter

Diekmann, Stephan

机构：

[1] Leibniz Inst Agr Res, Fritz Lipmann Inst, D-07745 Jena, Germany

[2] Albert Einstein Coll Med, Dept Pathol, Bronx, NY 10416 USA

来源：

BIOLOGICAL CHEMISTRY | 2006年 / 387卷 / 09期

关键词：

Alzheimer's disease; amyloid; cholesterol; hpTLC; lipid rafts; paired helical filaments; prion;

D O I：

10.1515/BC.2006.157

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

By using qualitative and quantitative high-performance thin layer chromatography (hpTLC) we found lipids associated with purified Alzheimer's (AD) paired helical filaments (PHF) in an amount of 1.4 +/- 0.2% of the total anhydrous mass. Compared to normal brain tissue these lipids have an unusual lipid class composition. The most prominent lipid classes were phosphatidylcholine (PC), cholesterol (CH), galactocerebrosides (GC) and sphingomyelin (SM). In addition, the use of micro high-performance liquid chromatography (HPLC) in combination with matrix-assisted laser desorption and ionisation time-of-flight mass spectrometry (MALDI-TOF-MS) allowed the determination of the molecular species of the polar membrane lipid classes present in PHF. The lipid pattern of intracellular PHF shows many characteristics of the conserved lipid pattern previously described for extracellular amyloid fibrils, suggesting similarities in their pathway of formation.

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页码：1267 / 1274

页数：8

相关论文

共 42 条

[1] Globular amyloid β-peptide1-42 oligomer -: a homogenous and stable neuropathological protein in Alzheimer's disease [J].

Barghorn, S ;

Nimmrich, V ;

Striebinger, A ;

Krantz, C ;

Keller, P ;

Janson, B ;

Bahr, M ;

Schmidt, M ;

Bitner, RS ;

Harlan, J ;

Barlow, E ;

Ebert, U ;

Hillen, H .

JOURNAL OF NEUROCHEMISTRY, 2005, 95 (03) :834-847

[2] Toward a unified scheme for the aggregation of tau into Alzheimer paired helical filaments [J].

Barghorn, S ;

Mandelkow, E .

BIOCHEMISTRY, 2002, 41 (50) :14885-14896

[3] Conversion of raft associated prion protein to the protease-resistant state requires insertion of PrP-res (PrPSc) into contiguous membranes [J].

Baron, GS ;

Wehrly, K ;

Dorward, DW ;

Chesebro, B ;

Caughey, B .

EMBO JOURNAL, 2002, 21 (05) :1031-1040

[4] Alzheimer's disease - Tangle disentanglement [J].

Beyreuther, K ;

Masters, CL .

NATURE, 1996, 383 (6600) :476-477

[5] Two types of Alzheimer's β-amyloid (1-40) peptide membrane interactions:: Aggregation preventing transmembrane anchoring Versus accelerated surface fibril formation [J].

Bokvist, M ;

Lindström, F ;

Watts, A ;

Gröbner, G .

JOURNAL OF MOLECULAR BIOLOGY, 2004, 335 (04) :1039-1049

[6] Convergence of atherosclerosis and Alzheimer's disease: inflammation, cholesterol, and misfolded proteins [J].

Casserly, I ;

Topol, E .

LANCET, 2004, 363 (9415) :1139-1146

[7] Abnormal tau-containing filaments in neurodegenerative diseases [J].

Crowther, RA ;

Goedert, M .

JOURNAL OF STRUCTURAL BIOLOGY, 2000, 130 (2-3) :271-279

[8] STRUCTURAL STUDIES ON FAP FIBRILS - REMOVAL OF CONTAMINANTS IS ESSENTIAL FOR THE INTERPRETATION OF X-RAY DATA [J].

DAMAS, A ;

SEBASTIAO, MP ;

DOMINGUES, FS ;

COSTA, PP ;

SARAIVA, MJ .

AMYLOID-INTERNATIONAL JOURNAL OF EXPERIMENTAL AND CLINICAL INVESTIGATION, 1995, 2 (03) :173-178

[9] Myoglobin forms amyloid fibrils by association of unfolded polypeptide segments [J].

Fändrich, M ;

Forge, V ;

Buder, K ;

Kittler, M ;

Dobson, CM ;

Diekmann, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (26) :15463-15468

[10] Raft lipids as common components of human extracellular amyloid fibrils [J].

Gellermann, GP ;

Appel, TR ;

Tannert, A ;

Radestock, A ;

Hortschansky, P ;

Schroeckh, V ;

Leisner, C ;

Lütkepohl, T ;

Shtrasburg, S ;

Röcken, C ;

Pras, M ;

Linke, RP ;

Diekmann, S ;

Fändrich, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (18) :6297-6302

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