An Improved Method to Knock Out the asd Gene of Salmonella enterica Serovar Pullorum

被引：17

作者：

Geng, Shi-Zhong ^{[1
]}

Jiao, Xin-An ^{[1
]}

Pan, Zhi-Ming ^{[1
]}

Chen, Xiao-Juan ^{[1
]}

Zhang, Xiao-Ming ^{[1
]}

Chen, Xiang ^{[1
]}

机构：

[1] Yangzhou Univ, Jiangsu Key Lab Zoonosis, Yangzhou 225009, Jiangsu, Peoples R China

来源：

JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY | 2009年

基金：

中国国家自然科学基金;

关键词：

POLYMERASE CHAIN-REACTION; GRAM-NEGATIVE BACTERIA; ESCHERICHIA-COLI; OLIGONUCLEOTIDE PRIMERS; ANTIBIOTIC-RESISTANCE; STABLE MAINTENANCE; VACCINE STRAINS; CLONING; TYPHIMURIUM; DISRUPTION;

D O I：

10.1155/2009/646380

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An asd-deleted (Delta asd) mutant of Salmonella enterica serovar Pullorum (SP) was constructed using an improved method of gene knockout by combining the pi-suicide plasmid system with the Red Disruption system. The asd gene was efficiently knocked out by the recombinant suicide vector, which replaced the asd gene with the Cm-R gene. Based on the balanced lethal host-vector system, the phenotype of the Delta asd mutant was further defined. The improved method was simpler and more effective than previously reported conventional methods.

引用

页数：8

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