Bioorthogonal Chemistry: Fishing for Selectivity in a Sea of Functionality

被引：2422

作者：

Sletten, Ellen M. ^{[1
]}

Bertozzi, Carolyn R. ^{[1
,2
,3
,4
]}

机构：

[1] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA

[2] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA

[3] Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USA

[4] Lawrence Berkeley Natl Lab, Berkeley, CA 94720 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2009年 / 48卷 / 38期

关键词：

alkynes; azides; bioconjugation; bioorthogonal reactions; Staudinger ligation; SITE-SPECIFIC INCORPORATION; TRANSFER-RNA-SYNTHETASE; CELL-SURFACE-PROTEINS; UNNATURAL AMINO-ACIDS; TRACELESS STAUDINGER LIGATION; AZIDE-ALKYNE CYCLOADDITION; GLCNAC-MODIFIED PROTEINS; NEWLY SYNTHESIZED PROTEINS; GREEN FLUORESCENT PROTEIN; LANTHANIDE-BINDING TAGS;

D O I：

10.1002/anie.200900942

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The study of biomolecules in their native environments is a challenging task because of the vast complexity of cellular systems. Technologies developed in the last few years for the selective modification of biological species in living systems have yielded new insights into cellular processes. Key to these new techniques are bioorthogonal chemical reactions, whose components must react rapidly and selectively with each other under physiological conditions in the presence of the plethora of functionality necessary to sustain life. Herein we describe the bioorthogonal chemical reactions developed to date and how they can be used to study biomolecules. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：6974 / 6998

页数：25

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