Production and biochemical characterization of an α-amylase from the moderate halophile Halomonas meridiana

被引:36
作者
Coronado, MJ
Vargas, C
Hofemeister, J
Ventosa, A
Nieto, JJ [1 ]
机构
[1] Univ Seville, Fac Pharm, Dept Microbiol & Parasitol, Seville 41012, Spain
[2] Inst Pflanzengenet & Kulturpflanzenforsch, D-06466 Gatersleben, Germany
关键词
moderate halophile; amylase production; Halomonas meridiana;
D O I
10.1016/S0378-1097(99)00628-X
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Extracellular amylase production by the moderate halophile Halomonas meridiana was optimized and the enzyme was characterized biochemically. The highest amylase production was achieved by growing H. meridiana cultures in media with 5% salts and starch, in the absence of glucose until the end of the exponential phase. The amylase exhibited maximal activity at pH 7.0, being relatively stable in alkaline conditions. Optimal temperature and salinity for activity were 37 degrees C and 10%, NaCl, respectively. Moreover, activity at salinity as high as 30% salts was detected. Maltose and maltotriose were the main end products of starch hydrolysis, indicating an alpha-amylase activity. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码:67 / 71
页数:5
相关论文
共 24 条
[1]   Enzymes from microorganisms in extreme environments [J].
Adams, MWW ;
Kelly, RM .
CHEMICAL & ENGINEERING NEWS, 1995, 73 (51) :32-42
[2]  
[Anonymous], 1996, Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology
[3]  
Antranikian G., 1992, Microbial degradation of natural products., P27
[4]  
CHAMBLISS GH, 1992, BACILLUS SUBTILIS OT, P213
[5]  
COHEN G N, 1956, Ann Inst Pasteur (Paris), V91, P693
[6]  
Cowan ST, 1974, COWAN STEELS MANUAL
[7]   DETERMINATION OF REDUCING SUGAR WITH IMPROVED PRECISION [J].
DYGERT, S ;
LI, LH ;
FLORIDA, D ;
THOMA, JA .
ANALYTICAL BIOCHEMISTRY, 1965, 13 (03) :367-&
[8]  
FERRARI E, 1992, BACILLUS SUBTILIS OT, P213
[9]   COMPATIBLE SOLUTES OF HALOPHILIC EUBACTERIA - MOLECULAR PRINCIPLES, WATER-SOLUTE INTERACTION, STRESS PROTECTION [J].
GALINSKI, EA .
EXPERIENTIA, 1993, 49 (6-7) :487-496
[10]  
HENGGEARONIS R, 1996, ESCHERICHIA COLI SAL, P1325