Production of bioherbicide by Phoma sp in a stirred-tank bioreactor

The objective of this work was to produce an herbicide by submerged fermentation in a stirred-tank bioreactor and to assess the potential herbicidal in pre-emergence, post-emergence, and in a detached leaves of Cucumis sativus var species. wisconsin (cucumber) and Sorghum bicolor (sorghum) species. Fermentations were carried out in a stirred-tank bioreactor with useful volume of 3L. Stirring rate (40, 50, and 60 rpm) and aeration (1, 2 and 3 vvm) were the variables studied for bioherbicide production. Fermented broth was fractioned with different solvents to identify the molecules produced by the fungus in a multi-dimensional gas chromatograph system. Bioherbicide showed 100% inhibition of germination of both species in the pre-emergence tests. From detached leaves tests were verified yellowish lesions in Cucumis sativus and necrotic lesions on leaves of Sorghum bicolor. Post-emergence test presented variation of the phytotoxicity from 25 to 66% for the species C. sativus and from 32 to 58% by S. bicolor. The metabolites produced by submerged fermentation of Phoma sp. presented activity in pre-emergence, post-emergence, and detached leaves of C. sativus and S. bicolor and it could be an alternative in the future for weed control.