Modulation of bft expression by the Bacteroides fragilis pathogenicity island and its flanking region

被引：22

作者：

Franco, AA

Cheng, RK

Goodman, A

Sears, CL

机构：

[1] Johns Hopkins Univ, Dept Med, Div Infect Dis, Baltimore, MD 21205 USA

[2] Johns Hopkins Univ, Dept Med, Div Gastroenterol, Baltimore, MD 21205 USA

来源：

MOLECULAR MICROBIOLOGY | 2002年 / 45卷 / 04期

关键词：

D O I：

10.1046/j.1365-2958.2002.03077.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To establish a recombinant system for high-level expression of biologically active Bacteroides fragilis toxin (BFT), we studied the expression of bft in non-toxigenic B. fragilis (NTBF) strains. The bft gene and the B. fragilis pathogenicity island (BfPAI) were cloned into NTBF strains with two distinct genetic patterns: (i) pattern II, strains lacking the BfPAI and its flanking region; and (ii) pattern III, strains lacking the BfPAI but containing its flanking region. Analysis of BFT activity of these recombinant strains on HT29/C1 cells showed that both the BfPAI and its flanking regions are important to optimal BFT activity. Reverse transcription polymerase chain reaction (RT-PCR) analysis indicated that the BfPAI and its flanking regions modulate bft expression. Further experiments demonstrated that the approximate to700 bp region upstream of bft is the BfPAI region critical for optimal bft expression. We conclude that both the region flanking the BfPAI and approximate to700 bp region upstream of bft are crucial to maximal BFT production by ETBF strains.

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收藏

页码：1067 / 1077

页数：11

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