A universal and rapid protocol for protein extraction from recalcitrant plant tissues for proteomic analysis

被引：435

作者：

Wang, Wei ^{[1
]}

Vignani, Rita ^{[1
]}

Scali, Monica ^{[1
]}

Cresti, Mauro ^{[1
]}

机构：

[1] Univ Siena, Dipartimento Sci Ambientali G Sarfatti, I-53100 Siena, Italy

来源：

ELECTROPHORESIS | 2006年 / 27卷 / 13期

关键词：

plant tissue; proteomics; sample preparation; two-dimensional electrophoresis;

D O I：

10.1002/elps.200500722

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple and universally applicable protocol for extracting high-quality proteins from recalcitrant plant tissues is described. We have used the protocol with no modification, for a wide range of leaves and fruits. In all cases, this protocol allows to obtain good electrophoretic separation of proteins. As the protocol is rapid, universal, and compatible with silver staining, it could be used for routine protein extraction from recalcitrant plant tissues for proteomic analysis.

引用

页码：2782 / 2786

页数：5

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