A panel of monoclonal antibodies against human polycomb group proteins

被引:28
作者
Hamer, KM [1 ]
Sewalt, RGAB [1 ]
Den Blaauwen, JL [1 ]
Hendrix, T [1 ]
Satijn, DPE [1 ]
Otte, AP [1 ]
机构
[1] Univ Amsterdam, Bioctr, Swammerdam Inst Life Sci, NL-1018 TV Amsterdam, Netherlands
来源
HYBRIDOMA AND HYBRIDOMICS | 2002年 / 21卷 / 04期
关键词
D O I
10.1089/153685902760213859
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Polycomb-group (PcG) proteins are chromatin-associated proteins that heritably repress gene activity in many organisms, including man. Two distinct human PcG complexes have been identified. The HPC/HPH PcG complex I contains the HPC, HPH, RING1, and BMI1 proteins, the EED/EZH2 PcG complex 11 contains the EED, EZH2, and YY1 proteins. Previously we found that the relative expression levels of proteins of the human PcG complexes I and 11 are severely deregulated in human tumors. These findings signify an important role for antibodies against human PcG proteins as diagnostic tools. To be able to produce standardized anti-human PcG antibodies, we developed a panel of five mouse monoclonal antibodies (MAbs) against the human PcG proteins HPC2, BMI1, RING1A, EED, and EZH2. All MAbs can be used for Western blot analysis and immunofluorescence labeling of tissue culture cells. With the exception of the MAb against HPC2, all MAbs can also be used in immunoprecipitation experiments and immunohistochemistry of human tissues. The novel MAbs are therefore valuable tools for the cell biological, biochemical, and pathological analysis of human PcG proteins.
引用
收藏
页码:245 / 252
页数:8
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