Human monocytes activate porcine endothelial cells, resulting in increased E-selectin, interleukin-8, monocyte chemotactic protein-1, and plasminogen activator inhibitor-type-1 expression

被引:62
作者
Millan, MT
Geczy, C
Stuhlmeier, KM
Goodman, DJ
Ferran, C
Bach, FH
机构
[1] HARVARD UNIV,SCH MED,DEACONESS HOSP,DEPT SURG,BOSTON,MA 02215
[2] HEART RES INST,CAMPERDOWN,NSW,AUSTRALIA
关键词
D O I
10.1097/00007890-199702150-00016
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Monocytes (Mo) are thought to be important effector cells in early xenograft rejection. Effects of Mo-endothelial cell (EC) interactions on EC activation in vitro were studied by coculturing human Mo or human monocytoid cell lines, U937 and THP-1, with porcine EC. Without preactivation, U937 cells and Mo induced mRNA for the EC-specific adhesion receptor, E-selectin, expressed only on activated cells, after 2 hr. Surface protein was maximal when equal numbers of EC and Mo were cocultured. Increased mRNA expression of the chemokines, interleukin-8 and monocyte chemotactic protein-1, and the antifibrinolytic protein plasminogen activator inhibitor type-1, confirmed EC activation. Like E-selectin, plasminogen activator inhibitor type-1 mRNA was rapidly induced and returned to baseline after 24 hr, whereas chemokine gene expression was slower and more prolonged. Interleukin-1 receptor antagonist failed to modulate induction of E-selectin. Soluble tumor necrosis factor (TNF) alpha receptor inhibited E-selectin induced by TNF alpha, but not by U931 cells, and mRNA and protein on EC in Mo-EC mixtures cocultured at 1:1 ratios were not significantly reduced. The TNF alpha inhibitor did reduce E-selectin expression (30-40%), as well as induced chemokine gene expression (80%), at higher Mo-EC ratios. Despite this, minimal TNF alpha was detectable in supernatants. These results, along with the transwell experiments that confirmed a requirement for Mo-EC contact, suggest that membrane-bound TNF alpha may be involved. Thus, Mo-EC interactions in the porcine to human combination activated several EC functions, suggesting that initial Mo contact with the vessel wall of a xenogeneic graft may promote leukocyte recruitment, inflammation, and maintenance of thrombus, resulting in eventual organ destruction.
引用
收藏
页码:421 / 429
页数:9
相关论文
共 56 条
[1]   STRUCTURAL RECOGNITION OF A NOVEL FIBRINOGEN GAMMA-CHAIN SEQUENCE(117-133) BY INTERCELLULAR-ADHESION MOLECULE-1 MEDIATES LEUKOCYTE-ENDOTHELIUM INTERACTION [J].
ALTIERI, DC ;
DUPERRAY, A ;
PLESCIA, J ;
THORNTON, GB ;
LANGUINO, LR .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (02) :696-699
[2]  
BAGGIOLINI M, 1994, ADV IMMUNOL, V55, P97
[3]   MONOCYTE ADHERENCE TO HUMAN VASCULAR ENDOTHELIUM [J].
BEEKHUIZEN, H ;
VANFURTH, R .
JOURNAL OF LEUKOCYTE BIOLOGY, 1993, 54 (04) :363-378
[4]  
BEEKHUIZEN H, 1990, J IMMUNOL, V145, P510
[5]   ENDOTHELIAL LEUKOCYTE ADHESION MOLECULE-1 - AN INDUCIBLE RECEPTOR FOR NEUTROPHILS RELATED TO COMPLEMENT REGULATORY PROTEINS AND LECTINS [J].
BEVILACQUA, MP ;
STENGELIN, S ;
GIMBRONE, MA ;
SEED, B .
SCIENCE, 1989, 243 (4895) :1160-1165
[6]   ACTIVATION OF INTRAGRAFT ENDOTHELIAL AND MONONUCLEAR-CELLS DURING DISCORDANT XENOGRAFT REJECTION [J].
BLAKELY, ML ;
VANDERWERF, WJ ;
BERNDT, MC ;
DALMASSO, AP ;
BACH, FH ;
HANCOCK, WW .
TRANSPLANTATION, 1994, 58 (10) :1059-1066
[7]  
BLAKELY ML, 1993, SURG FORUM, V49, P399
[8]  
CALDERON TM, 1994, LAB INVEST, V70, P836
[9]   SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].
CHOMCZYNSKI, P ;
SACCHI, N .
ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159
[10]   VLA-4 INTEGRIN CAN MEDIATE CD11 CD18-INDEPENDENT TRANSENDOTHELIAL MIGRATION OF HUMAN MONOCYTES [J].
CHULUYAN, HE ;
ISSEKUTZ, AC .
JOURNAL OF CLINICAL INVESTIGATION, 1993, 92 (06) :2768-2777