Continuous oxygen monitoring of mammalian cell growth on space shuttle mission STS-93 with a novel radioluminescent oxygen sensor

被引:14
作者
Reece, JS
Miller, MJ
Arnold, MA
Waterhouse, C
Delaplaine, T
Cohn, L
Cannon, T
机构
[1] Univ Iowa, Dept Chem, Iowa City, IA 52242 USA
[2] Univ Iowa, Dept Phys & Astron, Iowa City, IA 52242 USA
[3] Walter Reed Army Inst Res, Dept Resuscitat Med, Silver Spring, MD USA
关键词
oxygen sensors; optical sensors; ruthenium fluorescence quenching; radioluminescence; spaceflight monitoring; bioreactor monitoring;
D O I
10.1385/ABAB:104:1:1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A compact, flow-through oxygen sensor device based on luminescence quenching was used to monitor dissolved oxygen levels during mammalian cell growth on the STS-93 mission of the Columbia space shuttle. Excitation of an oxygen-sensitive ruthenium complex was provided by a radioluminescent light source (0.9 mm in diameter, 2.5 mm long), and the intensity of the resulting luminescence was measured by a simple photodiode detector. The use of radioluminescence for the excitation light source is a unique approach that provides many features important for long-term and remote monitoring applications. For the spaceflight experiment, human lung fibroblast cells (WI-38) were grown in hollow-fiber bioreactors. Oxygen concentration was measured in the flow path both before and after the bioreactor cartridge in order to gain information about the metabolism of the cells. The sensor was found to be nonperturbing to cell growth and withstood the challenging physical conditions of shuttle launch and landing while maintaining a stable calibration function. In addition, the sensor provided physically meaningful oxygen predictions.
引用
收藏
页码:1 / 11
页数:11
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