Resveratrol-type oligostilbenes from Iris clarkei antagonize 20-hydroxyecdysone action in the Drosophila melanogaster BII cell line

被引：30

作者：

Keckeis, K ^{[1
]}

Sarker, SD ^{[1
]}

Dinan, L ^{[1
]}

机构：

[1] Univ Exeter, Hatherly Labs, Dept Biol Sci, Exeter EX4 4PS, Devon, England

来源：

CELLULAR AND MOLECULAR LIFE SCIENCES | 2000年 / 57卷 / 02期

关键词：

antagonist; resveratrol; stilbene; ecdysteroid; Iris clarkei; Iridaceae; receptor; steroid hormone;

D O I：

10.1007/PL00000694

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bioassay-guided high-performance liquid chromatography analysis of a MeOH extract of Iris clarkei seeds yielded the resveratrol-type oligomeric stilbenes, ampelopsin B and alpha-viniferin, which antagonize the action of 20-hydroxyecdysone; with a 20-hydroxyecdysone concentration of 50 nM, the ED50 values were 33 mu M and 10 mu M, respectively. The structures of these compounds were determined by spectroscopic analysis, notably ultraviolet, liquid secondary ion mass spectrometry and modern one- and two-dimensional nuclear magnetic resonance techniques.

引用

页码：333 / 336

页数：4

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