Decreased expression of long non-coding RNA WT1-AS promotes cell proliferation and invasion in gastric cancer

被引：57

作者：

Du, Tao ^{[1
]}

Zhang, Baogui ^{[2
,3
]}

Zhang, Shun ^{[1
]}

Jiang, Xiaohua ^{[1
]}

Zheng, Ping ^{[1
]}

Li, Jianfang ^{[3
]}

Yan, Min ^{[3
]}

Zhu, Zhenggang ^{[3
]}

Liu, Bingya ^{[3
]}

机构：

[1] Tongji Univ, Sch Med, Shanghai East Hosp, Dept Gastrointestinal Surg, Shanghai 200092, Peoples R China

[2] Jining Med Univ, Affiliated Hosp, Jining 272000, Peoples R China

[3] Shanghai Jiao Tong Univ, Sch Med, Shanghai Key Lab Gastr Neoplasms, Shanghai Inst Digest Surg,Dept Surg,Ruijin Hosp, Shanghai 200025, Peoples R China

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE | 2016年 / 1862卷 / 01期

基金：

中国国家自然科学基金;

关键词：

WT1-AS; Gastric cancer; LncRNA; Proliferation; Progression; X-CHROMOSOME; METHYLATION; METASTASIS; HOTAIR; TUMOR; PATHWAY; IDENTIFICATION; COMPLEXES; CARCINOMA; REVEALS;

D O I：

10.1016/j.bbadis.2015.10.001

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Tumor recurrence and metastasis remain the major obstacles for the successful treatment of patients diagnosed with gastric cancer. In recent years, long non-coding RNAs (lncRNAs) have been considered as key regulators of tumor behavior. In this study, we investigated the expression and biological role of a newly-identified cancer-related lncRNA, WT1-AS. We found that WT1-AS expression was significantly down-regulated in tumor tissues compared to matched adjacent non-tumor tissues. The WT1-AS expression level was also associated with tumor size and the clinicopathological stage. Cell proliferation, migration, and invasion were inhibited, and the proportion of G0/G1 cells increased when WT1-AS was ectopically-expressed in gastric cancer cells. Furthermore, ectopic expression of WT1-AS was demonstrated to inhibit tumor growth and metastasis in vivo. Finally, we found that WT1-AS overexpression could decrease ERK protein phosphorylation. Our study indicates that WT1-AS is significantly down-regulated in gastric cancers and may be correlated with tumor progression. (C) 2015 Published by Elsevier B.V.

引用

页码：12 / 19

页数：8

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