Acute exercise mobilises CD8+T lymphocytes exhibiting an effector-memory phenotype

被引:167
作者
Campbell, John P. [1 ]
Riddell, Natalie E. [1 ]
Burns, Victoria E. [1 ]
Turner, Mark [1 ]
van Zanten, Jet J. C. S. Veldhuijzen [1 ]
Drayson, Mark T. [2 ]
Bosch, Jos A. [1 ,3 ]
机构
[1] Univ Birmingham, Behav Med Grp, Sch Sport & Exercise Sci, Birmingham, W Midlands, England
[2] Univ Birmingham, Sch Med, Div Immun & Infect, Birmingham, W Midlands, England
[3] Univ Heidelberg, Mannheim Inst Publ Hlth Social & Prevent Med, Mannheim Med Fac, D-6900 Heidelberg, Germany
关键词
CCR7; CD27; Cytotoxic T lymphocytes; Exercise; Natural killer cells; Trafficking; ADHESION MOLECULE EXPRESSION; CD8(+) T-CELLS; PERIPHERAL-BLOOD COMPARTMENT; ACUTE PSYCHOLOGICAL STRESS; BETA-ADRENERGIC-RECEPTORS; HIGH-INTENSITY EXERCISE; NATURAL-KILLER-CELLS; IN-VITRO; EXHAUSTIVE EXERCISE; ENDURANCE EXERCISE;
D O I
10.1016/j.bbi.2009.02.011
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
An acute bout of exercise evokes mobilisation of lymphocytes into the bloodstream, which can be largely attributed to increases in CD8+ T lymphocytes (CD8TLs) and natural killer (NK) cells. Evidence further suggests that, even within these lymphocyte subsets, there is preferential mobilisation of cells that share certain functional and phenotypic characteristics, such as high cytotoxicity, low proliferative ability, and high tissue-migrating potential. These features are characteristic of effector-memory CD8TL subsets. The current study therefore investigated the effect of exercise on these newly-identified subsets. Thirteen healthy and physically active males (mean +/- SD: age 20.9 +/- 1.5 yr) attended three sessions: a control session (no exercise); cycling at 35% Watt(max) (low intensity exercise); and 85% Watt(max) (high intensity exercise). Each bout lasted 20 min. Blood samples were obtained before exercise, during the final min of exercise, and +15, and +60 min post-exercise. CD8TLs were classified into naive, central memory (CM), effector-memory (EM), and CD45RA+ effector-memory (RAEM) using combinations of the cell surface markers CCR7, CD27, CD62L, CD57, and CD45RA. In parallel, the phenotypically distinct CD56(bright) 'regulatory' and CD56(dim) 'cytotoxic' NK subsets were quantified. The results show a strong differential mobilisation of CD8TL subsets (RAEM > EM > CM > naive); during high intensity exercise the greatest increase was observed for RAEM CD8Tls (+450%) and the smallest for naive cells (+84%). Similarly, CD56(dim) NK cells (+995%) were mobilised to a greater extent than CD56(bright) (+153%) NK cells. In conclusion, memory CD8TL that exhibit a high effector and tissue-migrating potential are preferentially mobilised during exercise. This finding unifies a range of independent observations regarding exercise-induced phenotypic and functional changes in circulating lymphocytes. The selective mobilisation of cytotoxic tissue-migrating subsets, both within the NK and CD8TL population, may enhance immune-surveillance during exercise. (C) 2009 Elsevier Inc. All rights reserved.
引用
收藏
页码:767 / 775
页数:9
相关论文
共 64 条
[1]  
[Anonymous], 1893, DEUT ARCH KLIN MED
[2]  
Astrand P., 2003, Textbook of work physiology, V4th, P273
[3]   Acute psychological stress alerts the adaptive immune response: Stress-induced mobilization of effector T cells [J].
Atanackovic, Djordje ;
Schnee, Benjamin ;
Schuch, Gunter ;
Faltz, Christiane ;
Schulze, Julia ;
Weber, Cora S. ;
Schafhausen, Philippe ;
Bartels, Katrin ;
Bokemeyer, Carsten ;
Brunner-Weinzierl, Monika Christine ;
Deter, Hans-Christian .
JOURNAL OF NEUROIMMUNOLOGY, 2006, 176 (1-2) :141-152
[4]   THE EFFECTS OF BETA-ADRENOCEPTOR STIMULATION ON ADHESION OF HUMAN NATURAL-KILLER-CELLS TO CULTURED ENDOTHELIUM [J].
BENSCHOP, RJ ;
NIJKAMP, FP ;
BALLIEUX, RE ;
HEIJNEN, CJ .
BRITISH JOURNAL OF PHARMACOLOGY, 1994, 113 (04) :1311-1316
[5]  
Borg G., 1998, Borgs Perceived Exertion and Pain Scales, P39
[6]   Differential mobilization of functionally distinct natural killer subsets during acute psychologic stress [J].
Bosch, JA ;
Bernston, GG ;
Cacioppo, JT ;
Marucha, PT .
PSYCHOSOMATIC MEDICINE, 2005, 67 (03) :366-375
[7]   Expression of CD57 defines replicative senescence and antigen-induced apoptotic death of CD8+ T cells [J].
Brenchley, JM ;
Karandikar, NJ ;
Betts, MR ;
Ambrozak, DR ;
Hill, BJ ;
Crotty, LE ;
Casazza, JP ;
Kuruppu, J ;
Migueles, SA ;
Connors, M ;
Roederer, M ;
Douek, DC ;
Koup, RA .
BLOOD, 2003, 101 (07) :2711-2720
[8]   Exercise induces recruitment of lymphocytes with an activated phenotype and short telomeres in young and elderly humans [J].
Bruunsgaard, H ;
Jensen, MS ;
Schjerling, P ;
Halkjær-Kristensen, J ;
Ogawa, K ;
Skinhoj, P ;
Pedersen, BK .
LIFE SCIENCES, 1999, 65 (24) :2623-2633
[9]   CCR7 expression and memory T cell diversity in humans [J].
Campbell, JJ ;
Murphy, KE ;
Kunkel, EJ ;
Brightling, CE ;
Soler, D ;
Shen, ZM ;
Boisvert, J ;
Greenberg, HB ;
Vierra, MA ;
Goodman, SB ;
Genovese, MC ;
Wardlaw, AJ ;
Butcher, EC ;
Wu, LJ .
JOURNAL OF IMMUNOLOGY, 2001, 166 (02) :877-884
[10]   Total lymphocyte CD8 expression is not a reliable marker of cytotoxic T-cell populations in human peripheral blood following an acute bout of high-intensity exercise [J].
Campbell, John P. ;
Guy, Keith ;
Cosgrove, Cormac ;
Florida-James, Geraint D. ;
Simpson, Richard J. .
BRAIN BEHAVIOR AND IMMUNITY, 2008, 22 (03) :375-380