Establishment of monoclonal antibodies specific for Bacillus subtilis DB9011

被引：3

作者：

Asano, Y

Akaishi, E

Tajima, K

Shinozawa, T ^{[1
]}

机构：

[1] Gunma Univ, Fac Engn, Dept Biol & Chem Engn, Kiryu, Gumma 3768515, Japan

[2] AHC Inc, Maebashi, Gumma 3710831, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2000年 / 64卷 / 03期

关键词：

Bacillus subtilis DB9011; ELISA; flagellin; monoclonal antibody;

D O I：

10.1271/bbb.64.652

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bacillius subtilis DB9011 is a strain with useful functions for agriculture. To establish a method for the discrimination of this strain from others, monoclonal antibodies (MAbs) mere prepared. Although two established MAbs (MAb9B6 and MAb14D2) cross-react with some other Bacillus strains in ELISA, only B. subtilis DB9011 vegetative cells are recognized by both MAbs. MAb14D2 recognizes flagellin, a 34-kDa unit protein of flagella. The two MAbs established will provide powerful tools with which detailed analysis of this bacterial strain can be obtained under environmental conditions.

引用

页码：652 / 656

页数：5

共 26 条

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