The tomato RNA-directed RNA polymerase has no effect on gene silencing by RNA interference in transgenic mice

被引:7
作者
de Wit, T [1 ]
Grosveld, F [1 ]
Drabek, D [1 ]
机构
[1] Erasmus Univ, Fac Med, Dept Cell Biol & Genet, NL-3000 DR Rotterdam, Netherlands
关键词
double-stranded RNA (dsRNA); RNA interference (RNAi); RNA-directed RNA polymerase (RdRP); transgene;
D O I
10.1023/A:1015687008357
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Double-stranded RNA (dsRNA) has been shown to interfere with the function of specific genes in various invertebrate species. The application of dsRNA interference (RNAi) in vertebrates (zebrafish and mouse) is still limited to embryos and it is not clear whether the method is generally applicable. Using a transgenic mouse model we investigated whether a stably inherited dsRNA introduced as a transgene can interfere with the expression of a specific target gene in erythroid tissue during development. In our globin gene system we do not observe any specific RNA interference. We, therefore, also introduced another gene that may be involved in a mechanism of post transcriptional gene silencing (PTGS), namely RNA-dependent RNA polymerase (RdRP) that was proposed to be involved in producing RNAs that trigger PTGS in plants. However, even though the tomato RdRP is catalytically active in erythroid tissue, no RNAi was observed.
引用
收藏
页码:305 / 310
页数:6
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