A rice spotted leaf gene, SpI7, encodes a heat stress transcription factor protein

被引：247

作者：

Yamanouchi, U

Yano, M ^{[1
]}

Lin, HX

Ashikari, M

Yamada, K

机构：

[1] Inst Soc Technol Innovat Agr Forestry & Fisheries, Tsukuba, Ibaraki 3050854, Japan

[2] Natl Inst Agrobiol Sci, Dept Mol Genet, Tsukuba, Ibaraki 3058602, Japan

[3] Toyama Univ, Fac Sci, Dept Biol, Toyama 9308555, Japan

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2002年 / 99卷 / 11期

关键词：

D O I：

10.1073/pnas.112209199

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A rice spotted leaf (lesion-mimic) gene, SpI7, was identified by map-based cloning. High-resolution mapping with cleaved amplified polymorphic sequence markers enabled us to define a genomic region of 3 kb as a candidate for SpI7 We found one ORF that showed high similarity to a heat stress transcription factor (HSF). Transgenic analysis verified the function of the candidate gene for SpI7: leaf spot development was suppressed in spI7 mutants with a wild-type SpI7 transgene. Thus, we conclude that SpI7 encodes the HSF protein. The transcript of spI7 was observed in mutant plants. The levels of mRNAs (SpI7 in wild type and spI7 in mutant) increased under heat stress. Sequence analysis revealed only one base substitution in the HSF DNA-binding domain of the mutant allele, causing a change from tryptophan to cysteine.

引用

页码：7530 / 7535

页数：6

共 53 条

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