High-efficiency fluorescence quenching of conjugated polymers by proteins

被引：317

作者：

Fan, CH

Plaxco, KW

Heeger, AJ ^{[1
]}

机构：

[1] Univ Calif Santa Barbara, Dept Biochem & Chem, Inst Polymers & Organ Solids, Santa Barbara, CA 93106 USA

[2] Univ Calif Santa Barbara, Biomol Sci & Engn Program, Santa Barbara, CA 93106 USA

[3] Univ Calif Santa Barbara, Dept Phys, Santa Barbara, CA 93106 USA

[4] Univ Calif Santa Barbara, Dept Mat, Santa Barbara, CA 93106 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2002年 / 124卷 / 20期

关键词：

D O I：

10.1021/ja025899u

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The fluorescence of the water-soluble anionic conjugated polymer, poly[lithium 5-methoxy-2-(4-sulfobutoxy)-1,4-phenylenevinylene] (MBL-PPV), is quenched in dilute aqueous solution by cytochrome c, a small, naturally occurring electron-transfer protein. The large value obtained for the Stern-Volmer constant (Ksv = 3.2 × 108 at pH 7.4, and ̃109 in acidic solutions) is attributed to a combination of two factors: (1) facile ET between the luminescent semiconducting polymer and the protein and (2) the Columb attraction between the oppositely charged polyelectrolytes. This system shows significant potential for biosensor applications. Copyright © 2002 American Chemical Society.

引用

页码：5642 / 5643

页数：2

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