Altered functional loading causes differential effects in the subchondral bone and condylar cartilage in the temporomandibular joint from young mice

被引：85

作者：

Chen, J. ^{[1
]}

Sorensen, K. P. ^{[1
]}

Gupta, T. ^{[1
]}

Kilts, T. ^{[2
]}

Young, M. ^{[2
]}

Wadhwa, S. ^{[1
]}

机构：

[1] Univ Connecticut, Ctr Hlth, Sch Dent Med, Dept Craniofacial Sci,Div Orthodont, Farmington, CT 06030 USA

[2] NIDCR, Mol Biol Bones & Teeth Sect, Craniofacial & Skeletal Dis Branch, NIH,DHHS Bethesda, Bethesda, MD 20892 USA

来源：

OSTEOARTHRITIS AND CARTILAGE | 2009年 / 17卷 / 03期

关键词：

Temporomandibular joint; Mouse model; Mechanical loading; Fibrocartilage; Subchondral bone; MANDIBULAR CONDYLE; CHONDROCYTE MATURATION; RAT; GROWTH; PTHRP; OSTEOCLASTOGENESIS; EXPRESSION; DISORDERS; FORCES; RANKL;

D O I：

10.1016/j.joca.2008.05.021

中图分类号：

R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学（修复外科学）];

学科分类号：

100224 [整形外科学];

摘要：

Objective: Altered loading is an important etiological factor for temporomandibular joint (TMJ) disorders. Studies examining altered loading of the TMJ have been done in rats but the response of the TMJ to altered loading in mice is largely unknown. Therefore, due to the potential usefulness of genetically engineered mice, the goal of this study was to develop a mouse TMJ altered functional loading model. Methods: One hundred and thirty four, 21-day-old CD-1 female mice were divided into two groups: (1) normal loading (hard pellet diet) for 2-6 weeks and (2) altered functional loading (incisor trimming every other day and soft dough diet) for 2-6 weeks. The mandibular condylar cartilage was evaluated by histology, the subchondral bone was evaluated by microcomputed tomography (micro-CT) analysis and gene expression was evaluated by real time polymerase chain reaction (PCR) analysis. Results: Altered functional loading for 2-6 weeks caused significant reduction in the thickness of the condylar cartilage whereas, only at 4 weeks was there a significant decrease in the bone volume fraction and trabecular thickness of the subchondral bone. Gene expression analysis showed that altered functional loading for 4 weeks caused a significant reduction in the expression of SRY-box containing gene 9 (Sox9), Collagen type X (Col X), Indian hedgehog (Ihh), Collagen type 11 (Col II) and Vascular endothelial growth factor (Vegf) and altered loading for 6 weeks caused a significant decrease in the expression of Sox9, Col II, Vegf and Receptor activator of NF-kappa B ligand (Rankl compared to the normal loading group. Conclusion: Altered functional TMJ loading in mice for 2-6 weeks leads to a loss of the condylar cartilage and a transient loss in the density of the mandibular condylar subchondral bone. (c) 2008 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.

引用

页码：354 / 361

页数：8

共 23 条

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