A putative branched-chain-amino-acid transaminase gene required for HC-toxin biosynthesis and pathogenicity in Cochliobolus carbonum

被引：29

作者：

Cheng, YQ ^{[1
]}

Ahn, JH ^{[1
]}

Walton, JD ^{[1
]}

机构：

[1] Michigan State Univ, Dept Energy, Plant Res Lab, E Lansing, MI 48824 USA

来源：

MICROBIOLOGY-UK | 1999年 / 145卷

关键词：

Cochliobolus (Helminthosporium) carbonum; cyclic peptide; branched-chain-amino-acid transaminase; TOXF; HC-toxin;

D O I：

10.1099/00221287-145-12-3539

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The cyclic tetrapeptide HC-toxin is required for pathogenicity of the filamentous fungus Cochliobolus carbonum on maize. HC-toxin production is controlled by a complex locus, TOX2. The isolation and characterization of a new gene of the TOX2 locus, TOXF. is reported. It is shown that TOXF is specifically required for HC-toxin production and pathogenicity. It is present as two or three copies in all HC-toxin-producing (Tox2(+)) isolates and is absent in toxin-non-producing strains. The deduced amino acid sequence of TOXF has moderate homology to many known or putative branched-chain-amino-acid transaminases from various species. A strain of C. carbonum with all copies of TOXF disrupted grew normally but lost HC-toxin production and pathogenicity. It is proposed that TOXF has a biosynthetic role in HC-toxin synthesis, perhaps to aminate a precursor of Aeo (2-amino-9,10-epoxi-8-oxodecanoic acid).

引用

页码：3539 / 3546

页数：8

共 38 条

[1] Regulation of cyclic peptide biosynthesis and pathogenicity in Cochliobolus carbonum by TOXEp, a novel protein with a bZIP basic DNA-binding motif and four ankyrin repeats
Ahn, JH
Walton, JD
[J]. MOLECULAR AND GENERAL GENETICS, 1998, 260 (05): : 462 - 469
[2] A fatty acid synthase gene in Cochliobolus carbonum required for production of HC-toxin, cyclo(D-prolyl-L-alanyl-D-alanyl-L-2-Amino-9,10-epoxi-8-oxodecanoyl)
Ahn, JH
Walton, JD
[J]. MOLECULAR PLANT-MICROBE INTERACTIONS, 1997, 10 (02) : 207 - 214
[3] Chromosomal organization of TOX2, a complex locus controlling host-selective toxin biosynthesis in Cochliobolus carbonum
Ahn, JH
Walton, JD
[J]. PLANT CELL, 1996, 8 (05) : 887 - 897
[4] EVOLUTIONARY RELATIONSHIPS AMONG PYRIDOXAL-5'-PHOSPHATE-DEPENDENT ENZYMES - REGIO-SPECIFIC ALPHA-FAMILY, BETA-FAMILY, AND GAMMA-FAMILY
ALEXANDER, FW
SANDMEIER, E
MEHTA, PK
CHRISTEN, P
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1994, 219 (03): : 953 - 960
[5] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
Altschul, SF
Madden, TL
Schaffer, AA
Zhang, JH
Zhang, Z
Miller, W
Lipman, DJ
[J]. NUCLEIC ACIDS RESEARCH, 1997, 25 (17) : 3389 - 3402
[6] Twenty-five coregulated transcripts define a sterigmatocystin gene cluster in Aspergillus nidulans
Brown, DW
Yu, JH
Kelkar, HS
Fernandes, M
Nesbitt, TC
Keller, NP
Adams, TH
Leonard, TJ
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (04) : 1418 - 1422
[7] Carroll A.M., 1994, FUNGAL GENET NEWSL, V41, P22, DOI [DOI 10.4148/1941-4765.1367, 10.4148/19414765.1367, DOI 10.4148/19414765.1367]
[8] 13C labeling indicates that the epoxide-containing amino acid of HC-toxin is biosynthesized by head-to-tail condensation of acetate
Cheng, YQ
Le, LD
Walton, JD
Bishop, KD
[J]. JOURNAL OF NATURAL PRODUCTS, 1999, 62 (01): : 143 - 145
[9] Two yeast homologs of ECA39, a target for c-Myc regulation, code for cytosolic and mitochondrial branched-chain amino acid aminotransferases
Eden, A
Simchen, G
Benvenisty, N
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (34) : 20242 - 20245
[10] RAPID PRODUCTION OF FULL-LENGTH CDNAS FROM RARE TRANSCRIPTS - AMPLIFICATION USING A SINGLE GENE-SPECIFIC OLIGONUCLEOTIDE PRIMER
FROHMAN, MA
DUSH, MK
MARTIN, GR
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (23) : 8998 - 9002

← 1 2 3 4 →