Role of H164 in a unique dye-decolorizing heme peroxidase DyP

被引：24

作者：

Sugano, Y ^{[1
]}

Ishii, Y ^{[1
]}

Shoda, M ^{[1
]}

机构：

[1] Tokyo Inst Technol, Chem Resources Lab, Midori Ku, Yokohama, Kanagawa 2268503, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2004年 / 322卷 / 01期

关键词：

Thanatephorus cucumeris; peroxidase DyP; proximal histidine;

D O I：

10.1016/j.bbrc.2004.07.090

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The expression system of a unique dye-decolorizing peroxidase DyP in Escherichia coli has been constructed. The molecular mass of the expressed DyP (eDyP) is 47 kDa, indicating no any modification with saccharides. The characteristics of eDyP were almost the same as those of native DyP from a fungus Thanatephorus cucumeris Dec 1 and recombinant DyP with Aspergillus oryzae except thermostability. As H164 was suggested to be the proximal histidine based on the preliminary X-ray crystallographic analysis of DyP, the site-directed mutations H164A and H166A (residue near H164) were introduced into the gene encoding DyP. The specific activity and RZ value of the purified H164A were 1.52 U/mg and 0.11, respectively, which were 99.8% and 95% lower than those of eDyP, respectively. On the contrary, those of H166A were not different from those of eDyP. Therefore, H164 was confirmed to be the proximal histidine. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：126 / 132

页数：7

共 38 条

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