Babesia bovis rhoptry-associated protein 1 is immunodominant for T helper cells of immune cattle and contains T-cell epitopes conserved among geographically distant B-bovis strains

被引:48
作者
Brown, WC
McElwain, TF
Ruef, BJ
Suarez, CE
Shkap, V
ChitkoMcKown, CG
Tuo, WB
RiceFicht, AC
Palmer, GH
机构
[1] TEXAS A&M UNIV, DEPT VET PATHOL, COLLEGE STN, TX 77843 USA
[2] TEXAS A&M UNIV, DEPT MED BIOCHEM & GENET, COLLEGE STN, TX 77843 USA
[3] KIMRON VET INST, IL-50250 BET DAGAN, ISRAEL
关键词
D O I
10.1128/IAI.64.8.3341-3350.1996
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The ability of rhoptry-associated protein 1 (RAP-1) of Babesia bovis and Babesia bigemina to confer partial protective immunity in cattle has stimulated interest in characterizing both B-cell and T-cell epitopes of these proteins. It was previously shown that B. bovis RAP-1 associates with the merozoite surface as well as rhoptries and expresses B-cell epitopes conserved among otherwise antigenically different B. bovis strains. An aminoterminal 307-amino-acid domain of the molecule that is highly conserved in the B. bigemina RAP-1 homolog did not contain cross-reactive B-cell epitopes. The studies reported here demonstrate that B, bovis RAP-1 is strongly immunogenic for T helper (Th) cells from B. bovis-immune cattle and that like B-cell epitopes, Th-cell epitopes are conserved in different B. bovis strains but not in B. bigemina RAP-1. Lymphocytes from cattle immune to challenge with the Mexico strain of B, bovis proliferated against recombinant B. bovis RAP-1 protein derived from the Mexico strain, T-cell lines established by stimulating lymphocytes with recombinant RAP-I protein responded against B. bovis, but not B. bigemina, merozoites. T-cell lines established by repeated stimulation of lymphocytes with B. bovis membrane antigen proliferated strongly against RAP-1, demonstrating the immunodominant nature of this protein. RAP-1-specific CD4(+) T cell clones recognized Mexico, Texas, Australia, and Israel strains of B. bovis but neither B. bigemina merozoites nor recombinant B. bigemina RAP-1. Analysis of cytokine mRNA in RAP-1-specific Th cell clones revealed strong expression of gamma interferon but little or no expression of interleukin-2 (IL-2), IL-4, or IL-10. Gamma interferon production was confirmed by enzyme-linked immunosorbent assay, These results indicate the potential to use selected B, bovis RAP-1 peptides as immunogens to prime for strong, anamnestic, strain-cross-reactive type 1 immune responses upon exposure to B. bovis.
引用
收藏
页码:3341 / 3350
页数:10
相关论文
共 51 条
[1]   REVISITING AND REVISING SUPPRESSOR T-CELLS [J].
BLOOM, BR ;
SALGAME, P ;
DIAMOND, B .
IMMUNOLOGY TODAY, 1992, 13 (04) :131-136
[2]   IDENTIFICATION OF BABESIA-BOVIS MEROZOITE ANTIGENS SEPARATED BY CONTINUOUS-FLOW ELECTROPHORESIS THAT STIMULATE PROLIFERATION OF HELPER T-CELL CLONES DERIVED FROM B-BOVIS-IMMUNE CATTLE [J].
BROWN, WC ;
LOGAN, KS ;
ZHAO, SM ;
BERGMAN, DK ;
RICEFICHT, AC .
INFECTION AND IMMUNITY, 1995, 63 (08) :3106-3116
[3]   USE OF HELPER T-CELLS TO IDENTIFY POTENTIAL VACCINE ANTIGENS OF BABESIA-BOVIS [J].
BROWN, WC ;
RICEFICHT, AC .
PARASITOLOGY TODAY, 1994, 10 (04) :145-149
[4]   FUNCTIONAL AND PHENOTYPIC CHARACTERIZATION OF WC1(+)GAMMA/DELTA T-CELLS ISOLATED FROM BABESIA BOVIS-STIMULATED T-CELL LINES [J].
BROWN, WC ;
DAVIS, WC ;
CHOI, TSH ;
DOBBELAERE, DAE ;
SPLITTER, GA .
CELLULAR IMMUNOLOGY, 1994, 153 (01) :9-27
[5]   BABESIA-BOVIS - BOVINE HELPER T-CELL LINES REACTIVE WITH SOLUBLE AND MEMBRANE-ANTIGENS OF MEROZOITES [J].
BROWN, WC ;
LOGAN, KS .
EXPERIMENTAL PARASITOLOGY, 1992, 74 (02) :188-199
[6]   CELL-MEDIATED IMMUNE-RESPONSES TO BABESIA-BOVIS MEROZOITE ANTIGENS IN CATTLE FOLLOWING INFECTION WITH TICK-DERIVED OR CULTURED PARASITES [J].
BROWN, WC ;
LOGAN, KS ;
WAGNER, GG ;
TETZLAFF, CL .
INFECTION AND IMMUNITY, 1991, 59 (07) :2418-2426
[7]   HETEROGENEITY IN CYTOKINE PROFILES OF BABESIA-BOVIS-SPECIFIC BOVINE CD4+ T-CELL CLONES ACTIVATED IN-VITRO [J].
BROWN, WC ;
WOODS, VM ;
DOBBELAERE, DAE ;
LOGAN, KS .
INFECTION AND IMMUNITY, 1993, 61 (08) :3273-3281
[8]   IDENTIFICATION OF 2 TH1 CELL EPITOPES ON THE BABESIA-BOVIS-ENCODED 77-KILODALTON MEROZOITE PROTEIN (BB-1) BY USE OF TRUNCATED RECOMBINANT FUSION PROTEINS [J].
BROWN, WC ;
ZHAO, SM ;
WOODS, VM ;
TRIPP, CA ;
TETZLAFF, CL ;
HEUSSLER, VT ;
DOBBELAERE, DAE ;
RICEFICHT, AC .
INFECTION AND IMMUNITY, 1993, 61 (01) :236-244
[9]   BOVINE HELPER T-CELL CLONES RECOGNIZE 5 DISTINCT EPITOPES ON BABESIA-BOVIS MEROZOITE ANTIGENS [J].
BROWN, WC ;
ZHAO, SM ;
RICEFICHT, AC ;
LOGAN, KS ;
WOODS, VM .
INFECTION AND IMMUNITY, 1992, 60 (10) :4364-4372
[10]   BABESIA-BOVIS - CHARACTERIZATION OF THE T-HELPER CELL RESPONSE AGAINST THE 42-KDA MEROZOITE SURFACE-ANTIGEN (MSA-1) IN CATTLE [J].
BROWN, WC ;
PALMER, GH ;
MCELWAIN, TF ;
HINES, SA ;
DOBBELAERE, DAE .
EXPERIMENTAL PARASITOLOGY, 1993, 77 (01) :97-110