CGRP and adrenomedullin binding correlates with transcript levels for Calcitonin Receptor-Like Receptor (CRLR) and Receptor Activity Modifying Proteins (RAMPs) in rat tissues

被引：119

作者：

Chakravarty, P ^{[1
]}

Suthar, TP ^{[1
]}

Coppock, HA ^{[1
]}

Nicholl, CG ^{[1
]}

Bloom, SR ^{[1
]}

Legon, S ^{[1
]}

Smith, DM ^{[1
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Sch Med, Dept Metab Med, London W12 0NN, England

来源：

BRITISH JOURNAL OF PHARMACOLOGY | 2000年 / 130卷 / 01期

基金：

英国惠康基金;

关键词：

CGRP; adrenomedullin; CGRP receptor; adrenomedullin receptor; RAMP;

D O I：

10.1038/sj.bjp.0702975

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

1 Putative receptors for CGRP and adrenomedullin have been investigated in the rat. Calcitonin Receptor-Like Receptor (CRLR), in combination with Receptor Activity Modifying Proteins (RAMPs) is hypothesized to bind either CGRP or adrenomedullin. The receptors known as RDC1 and L1 have also been shown to bind CGRP and adrenomedullin respectively. 2 In this study it is shown that rat CRLR cDNA specifies a CGRP receptor when co-transfected with RAMP-1 cDNA and an adrenomedullin receptor when co-transfected with either RAMP-2 or RAMP-3 cDNA in human embryonic kidney 293 cells. 3 CRLR, RAMP, RCD1 and L1 mRNA levels and CGRP and adrenomedullin receptor densities have been measured and correlated with each other in eight rat tissues selected for their distinctive patterns of CGRP and adrenomedullin binding. 4 The data are consistent with the predictions of the CRLR/RAMP model. CGRP binding correlates well with RAMP-1 mRNA levels (R = 1.0, P = 0.007), adrenomedullin binding shows a tendency to vary with RAMP-2 mRNA levels (R = 0.85, P = 0.14) and total binding is correlated with CRLR mRNA levels (R = 0.94, P = 0.03). The data do not support the hypothesis that RDC1 and L1 account for the majority of CGRP and adrenomedullin binding respectively.

引用

页码：189 / 195

页数：7

共 29 条

[1] A cDNA encoding the calcitonin gene-related peptide type 1 receptor [J].