Perfect markers for the Rht-B1b and Rht-D1b dwarfing genes in wheat

被引:381
作者
Ellis, MH [1 ]
Spielmeyer, W [1 ]
Gale, KR [1 ]
Rebetzke, GJ [1 ]
Richards, RA [1 ]
机构
[1] CSIRO Plant Ind, Canberra, ACT 2601, Australia
关键词
wheat; reduced height; GA insensitivity; PCR markers;
D O I
10.1007/s00122-002-1048-4
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
PCR-based markers were developed to detect the point mutations responsible for the two major semi-dwarfing genes Rht-B1b (Rht1) and Rht-D1b (Rht2) in wheat. These markers were validated by testing 19 wheat varieties of known Rht genotype. They included Rht-B1b and Rht-D1b dwarfs, double-mutant varieties and tall wheats. These were correctly genotyped with the RhtB1b and Rht-D1b- specific primers, as well as markers specific for the tall alleles Rht-B1a and Rht-D1a. Using a family of doubled-haploid lines segregating for Rht-B1b and Rht-D1b, the markers were mapped to the expected homoeologous regions of chromosomes 4B and 4D, respectively. Both markers were strongly correlated with a reduction in height, accounting for 23% (Rht-B1b) and 44% (Rht-D1b) of the phenotypic variance in the population. These markers will have utility in marker-assisted selection of the Rht-B1b and Rht-D1b genes in wheat breeding programs.
引用
收藏
页码:1038 / 1042
页数:5
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