Staining paraffin embedded sections of scald of barley before paraffin removal

被引：3

作者：

Xi, K ^{[1
]}

Burnett, PA ^{[1
]}

机构：

[1] LETHBRIDGE RES CTR,LETHBRIDGE,AB T1J 4B1,CANADA

来源：

BIOTECHNIC & HISTOCHEMISTRY | 1997年 / 72卷 / 04期

基金：

加拿大自然科学与工程研究理事会;

关键词：

leaf infection; paraffin embedment; PAS reagent; Rhynchosporium secalis; scald of barley; seed infection;

D O I：

10.3109/10520299709082234

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Staining of paraffin embedded sections with periodic acid-Schiff reagent and fast green before paraffin removal resulted in differentiation of barley seed and]leaf tissue from fungal structures of Rhynchosrporium secalis. Crystal violet, toluidine blue O and aniline blue also successfully stained fungal! structures of R. secalis in barley leaf tissues. Staining of embedded sections before paraffin removal allows simple processing of a series of sections, saves time and reduces solvent consumption.

引用

页码：173 / 177

页数：5

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