Absence of viral interference and different susceptibility to interferon between hepatitis B virus and hepatitis C virus in human hepatocyte chimeric mice

被引：30

作者：

Hiraga, Nobuhiko ^{[1
,2
]}

Imamura, Michio ^{[1
,2
]}

Hatakeyama, Tsuyoshi ^{[1
]}

Kitamura, Shosuke ^{[1
]}

Mitsui, Fukiko ^{[1
]}

Tanaka, Shinji ^{[1
]}

Tsuge, Masataka ^{[1
,2
]}

Takahashi, Shoichi ^{[1
,2
]}

Abe, Hiromi ^{[2
,3
]}

Maekawa, Toshiro ^{[2
,3
]}

Ochi, Hidenori ^{[2
,3
]}

Tateno, Chise ^{[2
,4
]}

Yoshizato, Katsutoshi ^{[2
,4
,5
]}

Wakita, Takaji ^{[6
]}

Chayama, Kazuaki ^{[1
,2
,3
]}

机构：

[1] Hiroshima Univ, Grad Sch Biomed Sci, Dept Med & Mol Sci, Div Frontier Med Sci,Programs Biomed Res,Minami K, Hiroshima 7348551, Japan

[2] Hiroshima Univ, Liver Res Project Ctr, Hiroshima, Japan

[3] RIKEN, Inst Phys & Chem Res, SNP Res Ctr, Lab Liver Dis, Yokohama, Kanagawa, Japan

[4] PhoenixBio Co Ltd, Higashihiroshima, Japan

[5] Osaka City Univ, Grad Sch Med, Osaka 558, Japan

[6] Natl Inst Infect Dis, Dept Virol 2, Shinjuku Ku, Tokyo, Japan

来源：

JOURNAL OF HEPATOLOGY | 2009年 / 51卷 / 06期

关键词：

Superinfection; JFH-1; IFN-stimulated genes; GENOMIC ANALYSIS; RNA REPLICATION; HOST RESPONSE; LIVER; HCV; INFECTION; INHIBITION; EXPRESSION; RIBAVIRIN; REVEALS;

D O I：

10.1016/j.jhep.2009.09.002

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Background/Aims: Both hepatitis B virus (HBV) and hepatitis C virus (HCV) replicate in the liver and show resistance against innate immunity and interferon (IFN) treatment. Whether there is interference between these two viruses is still controversial. We investigated the interference between these two viruses and the mode of resistance against IFN. Methods: We performed infection experiments with either or both of the two hepatitis viruses in human hepatocyte chimeric mice. Huh7 cell lines with stable production of HBV were also established and transfected with HCV JFH1 clone. Mice and cell lines were treated with IFN. The viral levels in mice sera and culture supernatants and messenger RNA levels of IFN-stimulated genes were measured. Results: No apparent interference between the two viruses was seen ill vivo. Only a small (0.3 log) reduction in serum HBV and a rapid reduction in HCV were observed after IFN treatment, regardless of infection with the other virus. In ill vitro studies, no interference between the two viruses was observed. The effect of IFN on each virus was not affected by the presence of the other virus. IFN-induced reductions of viruses in culture supernatants were similar to those in ill vivo study. Conclusions: No interference between the two hepatitis viruses exists in the liver in the absence of hepatitis. The mechanisms of IFN resistance of the two viruses target different areas of the IFN system. (C) 2009 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved.

引用

页码：1046 / 1054

页数：9

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