Complete sequence of the large virulence plasmid pSFO157 of the sorbitol-fermenting enterohemorrhagic Escherichia coli O157:H- strain 3072/96

被引:38
作者
Brunder, Werner
Karch, Helge
Schmidt, Herbert
机构
[1] Univ Hohenheim, Inst Lebensmitteltechnol, D-70599 Stuttgart, Germany
[2] Univ Wurzburg, Inst Hyg & Mikrobiol, D-97070 Wurzburg, Germany
[3] Univ Munster, Inst Hyg, D-48149 Munster, Germany
关键词
enterohemorrhagic E. coli; O157; nonmotile; sorbitol-fermenting; plasmid; pSFO157; F-plasmid; transfer region; sfp fimbriae;
D O I
10.1016/j.ijmm.2006.05.005
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The large virulence plasmid pSFO157 of sorbitol-fermenting E coli O157:H- strain 3072/96 has a size of 121,239 bp and contains 96 open reading frames > 50 bp. It is therefore 29,162 bp larger (ca. 32%) than plasmid pO157 of E coli O157:H7 strain EDL933. Major differences between the plasmids are the absence of katP, espP, and toxB in pSFO157 and, instead of these, the presence of the sfp fimbriae gene cluster and a large part of an F-plasmid transfer region, the latter accounting for most of the additional DNA. The differences in the order of the genes and their composition, as well as the presence of a number of replication-associated genes and mobile genetic elements suggests that the large E. coli O 157 virulence plasmids have a complex evolutionary origin. (c) 2006 Elsevier GmbH. All rights reserved.
引用
收藏
页码:467 / 474
页数:8
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