Treatment of aqueous phenol with soybean peroxidase in the presence of polyethylene glycol

被引：95

作者：

Kinsley, C ^{[1
]}

Nicell, JA ^{[1
]}

机构：

[1] McGill Univ, Dept Civil Engn & Appl Mech, Montreal, PQ H3A 2K6, Canada

来源：

BIORESOURCE TECHNOLOGY | 2000年 / 73卷 / 02期

基金：

加拿大自然科学与工程研究理事会;

关键词：

additive; enzyme; waste treatment; soybean peroxidase; phenol; polyethylene glycol; polymerization; precipitation;

D O I：

10.1016/S0960-8524(99)00151-0

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Soybean peroxidase (SBP) catalyzes the oxidation and polymerization of aromatic compounds in the presence of hydrogen peroxide. The polymerized products precipitate from solution, providing an alternative to conventional treatment methods. Studies were conducted to characterize the use of polyethylene glycol (PEG) as an additive to increase the active life of the enzyme. The effectiveness of PEG increased with its molecular weight, with maximum protection accomplished with PEG of molecular weight 35,000. Linear relationships were found between the quantity of phenol to be treated (1.0-10 mM) and the optimum doses of SEP and PEG required for greater than 95% removal. Observations suggest that it is the interaction between the PEG and the polymeric products that results in the protection of SEP. Following treatment, approximately 25% of the optimum PEG dose remained in the supernatant. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：139 / 146

页数：8

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