A protein biosensor for lactate

被引：24

作者：

D'Auria, S

Gryczynski, Z

Gryczynski, I

Rossi, M

Lakowicz, JR

机构：

[1] Univ Maryland, Ctr Fluorescence Spect, Dept Biochem & Mol Biol, Baltimore, MD 21201 USA

[2] CNR, Inst Prot Biochem & Enzymol, I-80125 Naples, Italy

来源：

ANALYTICAL BIOCHEMISTRY | 2000年 / 283卷 / 01期

关键词：

D O I：

10.1006/abio.2000.4611

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Blood lactate is a clinically valuable diagnostic indicator. In this preliminary report we describe a protein biosensor for L-lactate based on beef heart lactate dehydrogenase (LDH). LDH was noncovalently labeled with 8-anilino-1-naphthalene sulfonic acid (ANS). The ANS-labeled LDH displayed an approximately 40% decrease in emission intensity upon binding lactate. This decrease can be used to measure the lactate concentration. The ANS-labeled LDH was further utilized in a new sensing format, polarization sensing, which is suitable for miniaturization to a point-of-care lactate monitor. However, temporal instability of beef heart LDH indicates the need for further protein engineering prior to development of a more robust lactatesensing protein. (C) 2000 Academic Press.

引用

页码：83 / 88

页数：6

共 35 条

[1] Serial blood lactate levels can predict the development of multiple organ failure following septic shock
Bakker, J
Gris, P
Coffernils, M
Kahn, RJ
Vincent, JL
[J]. AMERICAN JOURNAL OF SURGERY, 1996, 171 (02) : 221 - 226
[2] Burtis CA, 1999, TIETZ TXB CLIN CHEM, P1917
[3] The β-glycosidase from the hyperthermophilic archaeon Sulfolobus solfataricus:: enzyme activity and conformational dynamics at temperatures above 100°C
D'Auria, S
Nucci, R
Rossi, M
Gryczynski, I
Gryczynski, Z
Lakowicz, JR
[J]. BIOPHYSICAL CHEMISTRY, 1999, 81 (01) : 23 - 31
[4] Lactate blood levels in the perioperative period of orthotopic liver transplantation
DeGasperi, A
Mazza, E
Corti, A
Zoppi, F
Prosperi, M
Fantini, G
Scaiola, A
Colella, G
Amici, O
Notaro, P
Rocchini, A
Ceresa, F
Roselli, E
Grugni, MC
[J]. INTERNATIONAL JOURNAL OF CLINICAL & LABORATORY RESEARCH, 1997, 27 (02) : 123 - 128
[5] Intrinsic molecular fluorescence of lactate dehydrogenase: An analytical alternative for enzymic determination of pyruvate
deMarcos, S
Galban, J
Alonso, C
Castillo, JR
[J]. ANALYST, 1997, 122 (04) : 355 - 359
[6] DESHPANDE SA, 1997, DIS CHILDHOOD, V76, pF15
[7] Assessment of the lactate biosensor methodology
Detry, B
Nullens, W
Cao, ML
Frans, A
Robert, A
Clerbaux, T
[J]. EUROPEAN RESPIRATORY JOURNAL, 1998, 11 (01) : 183 - 187
[8] QUANTITATIVE-DETERMINATION OF THE ABSORPTION-SPECTRA OF CHROMOPHORES IN STRONGLY SCATTERING MEDIA - A LIGHT-EMITTING-DIODE BASED TECHNIQUE
FANTINI, S
FRANCESCHINI, MA
FISHKIN, JB
BARBIERI, B
GRATTON, E
[J]. APPLIED OPTICS, 1994, 33 (22): : 5204 - 5213
[9] Polarization sensing with visual detection
Gryczynski, I
Grycsynski, Z
Lakowicz, JR
[J]. ANALYTICAL CHEMISTRY, 1999, 71 (07) : 1241 - 1251
[10] GRYCZYNSKI Z, 2000, IN PRESS OPT ENG

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