Emergence of Bluetongue Serotypes in Europe, Part 2: The Occurrence of a BTV-11 Strain in Belgium

被引：55

作者：

De Clercq, K. ^{[1
]}

Mertens, P. ^{[2
]}

De Leeuw, I. ^{[1
]}

Oura, C. ^{[3
]}

Houdart, P. ^{[4
]}

Potgieter, A. C. ^{[5
]}

Maan, S. ^{[2
]}

Hooyberghs, J. ^{[4
]}

Batten, C. ^{[3
]}

Vandemeulebroucke, E. ^{[6
]}

Wright, I. M. ^{[5
]}

Maan, N. ^{[2
]}

Riocreux, F. ^{[7
]}

Sanders, A. ^{[3
]}

Vanderstede, Y. ^{[7
]}

Nomikou, K. ^{[2
]}

Raemaekers, M. ^{[4
]}

Bin-Tarif, A. ^{[3
]}

Shaw, A. ^{[2
]}

Henstock, M. ^{[3
]}

Breard, E. ^{[8
]}

Dubois, E. ^{[9
]}

Gastaldi-Thiery, C. ^{[9
]}

Zientara, S. ^{[8
]}

Verheyden, B. ^{[1
]}

Vandenbussche, F. ^{[6
]}

机构：

[1] Vet & Agrochem Res Ctr, Dept Virol, Sect Dev Diagnost Tools Epizoot Dis, Uccle, Belgium

[2] Inst Anim Hlth, Arbovirus Mol Res Grp, Pirbright Lab, Surrey, England

[3] Inst Anim Hlth, European Community Bluetongue Reference Lab, Pirbright Lab, Surrey, England

[4] Belgian Fed Agcy Safety Food Chain, Brussels, Belgium

[5] Onderstepoort Vet Inst, Div Virol, Onderstepoort, South Africa

[6] Vet & Agrochem Res Ctr, Dept Virol, Uccle, Belgium

[7] Vet & Agrochem Res Ctr, Coordinat Ctr Vet Diagnost CCDD, Uccle, Belgium

[8] AFSSA ENVA INRA, UMR 1161, Maisons Alfort, France

[9] AFSSA Site Sophia Antipolis, Unite Pathol Ruminants, Sophia Antipolis, France

来源：

TRANSBOUNDARY AND EMERGING DISEASES | 2009年 / 56卷 / 9-10期

基金：

英国生物技术与生命科学研究理事会;

关键词：

bluetongue virus; BTV-11; real-time RT-PCR; Belgium; modified live vaccine strain; TIME RT-PCR; VIRUS SEROTYPE-8; EPIDEMIC;

D O I：

10.1111/j.1865-1682.2009.01092.x

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

P>An EDTA-blood sample from a cow without clinical signs, which gave early birth to a newborn calf that died soon after delivery, was shown to be positive for bluetongue virus (BTV)-RNA using a group-specific real-time RT-PCR (RT-qPCR). In-house serotype-specific RT-qPCR assays for bluetongue virus serotype 1 (BTV-1), -6 and -8 all gave negative results. Subsequent assays were carried out using conventional (gel-based) RT-PCR primers for all 25 BTV serotypes and only two primer sets, both specific for BTV-11, gave bands of the expected size. The cDNAs generated were sequenced and comparisons of the genome segment 2 sequence with that of the modified 'live' vaccine strain of BTV-11 from South Africa showed 100% identity. A survey of all ruminants in a 1-km area around the first positive farm using a BTV-11 serotype-specific RT-qPCR revealed five other holdings with in total nine BTV-11 positive animals. A cross-sectional monitoring of dairy cattle in Belgium showed an overall prevalence of 3.8% on herd level and 0.2% on animal level. A BTV-11 has been introduced into the Belgian cattle herd during the 2008 vector season. The source of the infection and the way by which the virus was introduced are unknown.

引用

页码：355 / 361

页数：7

共 11 条

[1]

Cannon R.M., 1982, LIVESTOCK DIS SURVEY

[2] Transplacental infection and apparently immunotolerance induced by a wild-type bluetongue virus serotype 8 natural infection [J].

De Clercq, K. ;

De Leeuw, I. ;

Verheyden, B. ;

Vandemeulebroucke, E. ;

Vanbinst, T. ;

Herr, C. ;

Meroc, E. ;

Bertels, G. ;

Steurbaut, N. ;

Miry, C. ;

De Bleecker, K. ;

Maquet, G. ;

Bughin, J. ;

Saulmont, M. ;

Lebrun, M. ;

Sustronck, B. ;

De Deken, R. ;

Hooyberghs, J. ;

Houdart, P. ;

Raemaekers, M. ;

Mintiens, K. ;

Kerkhofs, P. ;

Goris, N. ;

Vandenbussche, F. .

TRANSBOUNDARY AND EMERGING DISEASES, 2008, 55 (08) :352-359

[3]

Dungu B, 2004, Vet Ital, V40, P616

[4] Establishing the spread of bluetongue virus at the end of the 2006 epidemic in Belgium [J].

Meroc, E. ;

Faes, C. ;

Herr, C. ;

Staubach, C. ;

Verheyden, B. ;

Vanbinst, T. ;

Vandenbussche, F. ;

Hooyberghs, J. ;

Aerts, A. ;

De Clercq, K. ;

Mintiens, K. .

VETERINARY MICROBIOLOGY, 2008, 131 (1-2) :133-144

[5]

*PROM MAIL, BLUET EUR 69 NETH BT

[6] Development and initial evaluation of a real-time RT-PCR assay to detect bluetongue virus genome segment 1 [J].

Shaw, A. E. ;

Monaghan, P. ;

Alpar, H. O. ;

Anthony, S. ;

Darpel, K. E. ;

Batten, C. A. ;

Guercio, A. ;

Alimena, G. ;

Vitale, M. ;

Bankowska, K. ;

Carpenter, S. ;

Jones, H. ;

Oura, C. A. L. ;

King, D. P. ;

Elliott, H. ;

Mellor, P. S. ;

Mertens, P. P. C. .

JOURNAL OF VIROLOGICAL METHODS, 2007, 145 (02) :115-126

[7] Bluetongue virus detection by two real-time RT-qPCRs targeting two different genomic segments [J].

Toussaint, J. F. ;

Sailleau, C. ;

Breard, E. ;

Zientara, S. ;

De Clercq, K. .

JOURNAL OF VIROLOGICAL METHODS, 2007, 140 (1-2) :115-123

[8] Bluetongue in Belgium, 2006 [J].

Toussaint, Jean-Francois ;

Sailleau, Corinne ;

Mast, Jan ;

Houdart, Philippe ;

Czaplicki, Guy ;

Demeestere, Lien ;

VandenBussche, Frank ;

van Dessel, Wesley ;

Goris, Nesya ;

Breard, Emmanuel ;

Bounaadja, Lotfi ;

Thiry, Etienne ;

Zientara, Stephan ;

De Clercq, Kris .

EMERGING INFECTIOUS DISEASES, 2007, 13 (04) :614-616

[9] Bluetongue Virus Detection by Real-Time RT-PCR in Culicoides Captured During the 2006 Epizootic in Belgium and Development of an Internal Control [J].

Vanbinst, T. ;

Vandenbussche, F. ;

Vandemeulebroucke, E. ;

De Leeuw, I. ;

Deblauwe, I. ;

De Deken, G. ;

Madder, M. ;

Haubruge, E. ;

Losson, B. ;

De Clercq, K. .

TRANSBOUNDARY AND EMERGING DISEASES, 2009, 56 (05) :170-177

[10] Evaluation of antibody-ELISA and real-time RT-PCR for the diagnosis and profiling of bluetongue virus serotype 8 during the epidemic in Belgium in 2006 [J].

Vandenbussche, Frank ;

Vanbinst, Tine ;

Verheyden, Bart ;

Van Dessel, Wesley ;

Derneestere, Lien ;

Houdart, Philippe ;

Bertels, Guido ;

Praet, Nicolas ;

Berkvens, Dirk ;

Mintiens, Koen ;

Goris, Nesya ;

De Clercq, Kris .

VETERINARY MICROBIOLOGY, 2008, 129 (1-2) :15-27

← 1 2 →