Detection of DNA damage and identification of UV-induced photoproducts using the CometAssay™ kit

被引：33

作者：

Lemay, M ^{[1
]}

Wood, KA ^{[1
]}

机构：

[1] Trevigen Inc, Gaithersburg, MD 20877 USA

来源：

BIOTECHNIQUES | 1999年 / 27卷 / 04期

关键词：

D O I：

10.2144/99274pf01

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We introduce the first commercially available comet assay for the detection and quantification of DNA damage in individual eukaryotic cells. The major difficulty Of the comet assay is the preparation of the slides needed to immobilize the samples throughout the lysis and electrophoretic procedure. The CometAssay(TM) kit uses a proprietary technology to precoat glass microscope slides to allow direct application of the agarose embedded sample without any additional slide treatment. in this report, Mle discuss the detection of DNA damage in individual cells exposed to ultraviolet irradiation using the new CometSlides(TM) and their cost compared to traditional slides.

引用

页码：846 / 851

页数：6

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