Microbial metabolism of evodiamine by Penicillium janthinellum and its application for metabolite identification in rat urine

被引：23

作者：

Li, Li

Liu, Rongxia

Ye, Min

Hu, Xinying

Wang, Qiao

Bi, Kaishun

Guo, Dean ^{[1
]}

机构：

[1] Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China

[2] Shenyang Pharmaceut Univ, Sch Pharmaceut Sci, Shenyang 110016, Peoples R China

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 2006年 / 39卷 / 04期

基金：

中国国家自然科学基金;

关键词：

evodiamine; metabolite; biotransformation; hydroxylation;

D O I：

10.1016/j.enzmictec.2005.10.029

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Microbial metabolism of evodiamine by Penicillium janthinellum AS 3.510 was investigated. Two metabolites were isolated from the broth and their structures were identified as 10-hydroxyevodiamine and 11-hydroxyevodiamine, by means of their IR, MS and NMR spectral data. Both of the metabolites were proved to be new compounds and showed strong cytotoxic activity in cytotoxicity assay. A major in vivo metabolite of evodiamine was detected in rat urine after oral administration of evodiamine and its structure was proved to be identical with that of the microbial metabolite 10-hydroxyevodiamine by HPLC-MS/MS analysis and comparison with the reference standard. The time course of microbial transformation of evodiamine and its urinary excretion in rat was proposed. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：561 / 567

页数：7

共 11 条

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