Water deficit triggers phospholipase D activity in the resurrection plant Craterostigma plantagineum

被引:171
作者
Frank, W
Munnik, T
Kerkmann, K
Salamini, F
Bartels, D
机构
[1] Max Planck Inst Zuchtungsforsch, D-50829 Cologne, Germany
[2] Fraunhofer Inst Umweltchem & Okotoxikol, Dept Mol Biotechnol, D-57392 Schmallenberg, Germany
[3] Univ Amsterdam, BioCentrum, Inst Mol Cell Biol, NL-1098 SM Amsterdam, Netherlands
[4] Max Delbruck Lab, D-50829 Cologne, Germany
[5] Univ Bonn, Inst Bot, D-53115 Bonn, Germany
关键词
D O I
10.1105/tpc.12.1.111
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Phospholipids play an important role in many signaling pathways in animal cells. Signaling cascades are triggered by the activation of phospholipid cleaving enzymes such as phospholipases C, D (PLD), and A(2). Their activities result in the formation of second messengers and amplification of the initial signal. In this study, we provide experimental evidence that PLD is involved in the early events of dehydration in the resurrection plant Craterostigma plantagineum. The enzymatic activity of the PLD protein was activated within minutes after the onset of dehydration, and although it was not inducible by abscisic acid, PLD activity did increase in response to mastoparan, which suggests a role for heterotrimeric G proteins in PLD regulation. Two cDNA clones encoding PLDs, CpPLD-1 and CpPLD-2, were isolated. The CpPLD-1 transcript was constitutively expressed, whereas CpPLD-2 was induced by dehydration and abscisic acid. Immunological studies revealed changes in the subcellular localization of the PLD protein in response to dehydration. Taken together, the data on enzymatic activity as well as transcript and protein distributions allowed us to propose a role for PLD in the events leading to desiccation tolerance in C. plantagineum.
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页码:111 / 123
页数:13
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