APOBEC-1 and AID are nucleo-cytoplasmic trafficking proteins but APOBEC3G cannot traffic

被引：27

作者：

Bennett, Ryan P.

Diner, Elie

Sowden, Mark P.

Lees, Joshua A.

Wedekind, Joseph E.

Smith, Harold C.

机构：

[1] Univ Rochester, Dept Biochem & Biophys, Rochester, NY 14642 USA

[2] Univ Rochester, James P Wilmot Ctr, Rochester, NY 14642 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2006年 / 350卷 / 01期

关键词：

APOBEC3G; AID; APOBEC-1; trafficking; NLS; NES;

D O I：

10.1016/j.bbrc.2006.09.032

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human APOBEC3G (hA3G) is a member of the APOBEC-1 related protein (ARP) family of cytidine deaminases. hA3G functions as a natural defense against endogenous retrotransposons and a multitude of retroviruses, most notably human immunodeficiency virus type I (HIV-1). Nothing is known about the cellular function of hA3G, however, upon HIV-1 infection hA3G functions as an antiviral factor by mutating viral single-stranded DNA during reverse transcription. Whereas homologous deaminases such as APOBEC-1 and AID act on RNA and DNA, respectively, in the cell nucleus, hA3G mutagenic activity appears to be restricted to the cytoplasm. We demonstrate that hA3G is not a nucleo-cytoplasmic shuttling protein like APOBEC-1 and AID, but is strongly retained in the cytoplasm through a mechanism that involves both the N and C-terminal regions of the protein. (c) 2006 Elsevier Inc. All rights reserved.

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页码：214 / 219

页数：6

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