Structure- and oil type-based efficacy of emulsion adjuvants

被引：34

作者：

Jansen, Theo ^{[1
]}

Hofmans, Marij P. M. ^{[1
]}

Theelen, Marc J. G. ^{[1
]}

Manders, Frans ^{[1
]}

Schijns, Virgil E. J. C. ^{[1
]}

机构：

[1] AkzoNobel, Serv Lab, Intervet Int BV, NL-5830 AA Boxmeer, Netherlands

来源：

VACCINE | 2006年 / 24卷 / 26期

关键词：

oil emulsions; vaccine; antibody responses; adjuvant; release;

D O I：

10.1016/j.vaccine.2006.03.074

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Oil-based emulsions are well-known immunopotentiators, for inactivated, "killed" vaccines. We addressed the relationship between emulsion structure and levels of in vivo antibody formation to inactivated New Castle Disease virus (NDV) and Infectious Bronchitis virus (IBV) as antigens in 3-week-old chickens. The use of a polymeric emulsifier allowed for direct comparison of three types of emulsions, water-in-oil (W/O), oil-in-water (O/W) and W/O-in-water (W/O/W), while maintaining an identical content of components for each vehicle. They were prepared with either non-metabolizable, mineral oil or metabolizable, Miglyol 840. In addition, we assessed the inherent release capacity of each emulsion variant in vitro. Remarkably, we noted that W/O-type emulsions induced the best immune responses, while they released no antigen during 3 weeks. In general, mineral oil vaccines showed superior efficacy compared to Miglyol 840-based vaccines. (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：5400 / 5405

页数：6

共 19 条

[1] Adjuvants designed for veterinary and human vaccines [J].

Aucouturier, J ;

Dupuis, L ;

Ganne, V .

VACCINE, 2001, 19 (17-19) :2666-2672

[2]

De Luka M., 1990, COSMIC TOILETRIES, V105, P65

[3]

Degen Winfried G J, 2003, Expert Rev Vaccines, V2, P327, DOI 10.1586/14760584.2.2.327

[4] EFFECTIVENESS OF LIPID AND LIPIDOPHILIC SUBSTANCES AS ADJUVANTS [J].

DRESSER, DW .

NATURE, 1961, 191 (479) :1169-&

[5] Dendritic cells internalize vaccine adjuvant after intramuscular injection [J].

Dupuis, M ;

Murphy, TJ ;

Higgins, D ;

Ugozzoli, M ;

van Nest, G ;

Ott, G ;

McDonald, DM .

CELLULAR IMMUNOLOGY, 1998, 186 (01) :18-27

[6]

Freund J, 1937, P SOC EXP BIOL MED, V37, P509

[7] Relationship between antigen release and antibody response of infectious coryza water-in-oil-in-water emulsion vaccines [J].

Fukanoki, S ;

Matsumoto, K ;

Mori, H ;

Takeda, R .

AVIAN DISEASES, 2000, 44 (04) :869-873

[8]

HERBERT WJ, 1968, IMMUNOLOGY, V14, P301

[9] Relationship between the degree of antigen adsorption to aluminum hydroxide adjuvant in interstitial fluid and antibody production [J].

Iyer, S ;

HogenEsch, H ;

Hem, SL .

VACCINE, 2003, 21 (11-12) :1219-1223

[10]

LEMOIGNIC, 1916, COMPT REND SOC BIOL, V79, P201

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