Epidermal powder immunization with a recombinant HIV gp120 targets Langerhans cells and induces enhanced immune responses

被引：24

作者：

Chen, D ^{[1
]}

Zuleger, C ^{[1
]}

Chu, Q ^{[1
]}

Maa, YF ^{[1
]}

Osorio, J ^{[1
]}

Payne, LG ^{[1
]}

机构：

[1] PowderJect Vaccines Inc, Madison, WI 53711 USA

来源：

AIDS RESEARCH AND HUMAN RETROVIRUSES | 2002年 / 18卷 / 10期

关键词：

D O I：

10.1089/088922202760072348

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The recombinant envelope gp120 (rgp120) of human immunodeficiency virus (HIV) is a weak immunogen when administered by intramuscular (IM) injection. In the present study, we report that epidermal powder immunization (EPI) elicits robust antibody responses to the rgp120. EPI of mice with a dose 0.2-5 mug of rgp120 protein elicited geometric mean antibody titers that were 18- to 240-fold higher than that elicited by IM injection using a 5.0 mug dose. Targeting antigen to and mobilization of Langerhans cells (LCs) by EPI may explain the enhanced immunogenicity of the rgp120. EPI with rgp120 using sugar and gold particles as carrier resulted in differential antigen entry into the LCs and differential IgG subclass antibody and cellular immune responses. EPI may serve as a useful tool to evaluate vaccine potential of the rgp120 protein.

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页码：715 / 722

页数：8

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