Array technology as a tool to monitor exposure of fish to xenoestrogens

被引:31
作者
Larkin, P
Folmar, LC
Hemmer, MJ
Poston, AJ
Lee, HS
Denslow, ND
机构
[1] Dept Biochem & Mol Biol, Gainesville, FL 32610 USA
[2] Ctr Biotechnol, Gainesville, FL 32610 USA
[3] US EPA, NHEERL, Gulf Ecol Div, Gulf Breeze, FL 32561 USA
关键词
biomarkers; endocrine disruption; estrogen; macroarray; mRNA expression; pollution monitoring; sheepshead minnow;
D O I
10.1016/S0141-1136(02)00172-1
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
A variety of anthropogenic chemicals are capable of binding to the estrogen receptor of vertebrate species. Binding of these compounds can interfere with homeostasis by disrupting normal gene expression patterns. The purpose of this study was to investigate the feasibility of applying array technology as a monitoring tool for detecting the presence and distribution of estrogenic compounds in coastal habitats using sheepshead minnows as our model. cDNA clones that were isolated from differential display, including vitellogenin alpha and beta, vitelline envelope protein (ZP2), and transferrin, among others, were spotted on the macroarray. The results of these experiments demonstrate a characteristic expression pattern of estrogen responsive genes in sheepshead minnows exposed to, 17 beta-estradiol (E-2). (C) 2002 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:395 / 399
页数:5
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