Three-dimensional imaging with optical tweezers

被引：42

作者：

Friese, MEJ ^{[1
]}

Truscott, AG ^{[1
]}

Rubinsztein-Dunlop, H ^{[1
]}

Heckenberg, NR ^{[1
]}

机构：

[1] Univ Queensland, Dept Phys, Ctr Laser Sci, Brisbane, Qld 4072, Australia

来源：

APPLIED OPTICS | 1999年 / 38卷 / 31期

关键词：

D O I：

10.1364/AO.38.006597

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We demonstrate a three-dimensional scanning probe microscope in which the extremely soft spring of an optical tweezers trap is used. Feedback control of the instrument based on backscattered light levels allows three-dimensional imaging of microscopic samples in an aqueous environment. Preliminary results with a 2-mu m-diameter spherical probe indicate that features of approximately 200 nm can be resolved, with a sensitivity of 5 nm in the height measurement. The theoretical resolution is limited by the probe dimensions. (C) 1999 Optical Society of America.

引用

页码：6597 / 6603

页数：7

共 10 条

[1] ATOMIC FORCE MICROSCOPE [J].

BINNIG, G ;

QUATE, CF ;

GERBER, C .

PHYSICAL REVIEW LETTERS, 1986, 56 (09) :930-933

[2] Determination of the force constant of a single-beam gradient trap by measurement of backscattered light [J].

Friese, MEJ ;

RubinszteinDunlop, H ;

Heckenberg, NR ;

Dearden, EW .

APPLIED OPTICS, 1996, 35 (36) :7112-7116

[3] SCANNING-FORCE MICROSCOPE BASED ON AN OPTICAL TRAP [J].

GHISLAIN, LP ;

WEBB, WW .

OPTICS LETTERS, 1993, 18 (19) :1678-1680

[4] MICROFABRICATED METAL AND OXIDE FIBERS FOR BIOLOGICAL APPLICATIONS [J].

GOLD, J ;

NILSSON, B ;

KASEMO, B .

JOURNAL OF VACUUM SCIENCE & TECHNOLOGY A, 1995, 13 (05) :2638-2644

[5] NEAR-FIELD SCANNING OPTICAL MICROSCOPE WITH A LASER TRAPPED PROBE [J].

KAWATA, S ;

INOUYE, Y ;

SUGIURA, T .

JAPANESE JOURNAL OF APPLIED PHYSICS PART 2-LETTERS & EXPRESS LETTERS, 1994, 33 (12A) :L1725-L1727

[6] TRAPPED-PARTICLE OPTICAL MICROSCOPY [J].

MALMQVIST, L ;

HERTZ, HM .

OPTICS COMMUNICATIONS, 1992, 94 (1-3) :19-24

[7] 2-COLOR TRAPPED-PARTICLE OPTICAL MICROSCOPY [J].

MALMQVIST, L ;

HERTZ, HM .

OPTICS LETTERS, 1994, 19 (12) :853-855

[8] OPTICAL STETHOSCOPY - IMAGE RECORDING WITH RESOLUTION LAMBDA/20 [J].

POHL, DW ;

DENK, W ;

LANZ, M .

APPLIED PHYSICS LETTERS, 1984, 44 (07) :651-653

[9]

Stout AL, 1998, METHOD CELL BIOL, V55, P99

[10] PARAMETRIC STUDY OF THE FORCES ON MICROSPHERES HELD BY OPTICAL TWEEZERS [J].

WRIGHT, WH ;

SONEK, GJ ;

BERNS, MW .

APPLIED OPTICS, 1994, 33 (09) :1735-1748

← 1 →