Cloning and characterization of the 5′-flanking region of the pig adiponectin gene

被引：21

作者：

Ling, Fei ^{[1
,2
]}

Lia, Jiaqi ^{[1
]}

Chen, Yaosheng ^{[3
]}

Du, Hongli ^{[2
]}

Mei, Yingjie ^{[1
]}

Mo, Delin ^{[3
]}

Wang, Chong ^{[1
]}

机构：

[1] S China Agr Univ, Coll Anim Sci, Guangzhou 510642, Guangdong, Peoples R China

[2] S China Univ Technol, Sch Biosci & Bioengn, Guangzhou 510640, Peoples R China

[3] Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol, Guangzhou 510006, Guangdong, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 381卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Adiponectin; Promoter; EMSA; CRE; 5 '-RACE; Pig; ADIPOSE-SPECIFIC GENE; 3T3-L1; ADIPOCYTES; TRANSCRIPTION INITIATION; INSULIN-RESISTANCE; GENOMIC STRUCTURE; APM-1; PROMOTER; EXPRESSION; PROTEIN; OBESITY; BETA;

D O I：

10.1016/j.bbrc.2009.02.031

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Adiponectin, a cytokine hormone secreted exclusively by adipose tissue, has key roles in energy homeostasis, and in glucose and lipid metabolism. To understand the regulatory expression of pig adiponectin, the T-flanking region of the adiponectin gene was isolated from a pig BAC library. 5'-RACE analysis revealed that there were three transcriptional start sites, including one that is novel. The luciferase reporter assay detected a positive cis-acting element for efficient expression of the adiponectin gene at the region spanned by nucleotides -1150 to -1130 with serially deleted 5'-flanking sequences as its promoters. Analysis Of oligonucleotide competition by the electrophoretic mobility-shift assay revealed the presence of a cAMP-responsive element (CRE) (nucleotides -1150 to -1130) for the cAMP-responsive element binding protein (CREB), which has not been reported in human or mouse adiponectin genes. These results indicated that CREB is an essential regulatory factor for the transcriptional activity of pig adiponectin. (C) 2009 Elsevier Inc. All rights reserved

引用

页码：236 / 240

页数：5

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