Novel, rapid purification of the membrane protein photosystem I by high-performance liquid chromatography on porous materials

被引：23

作者：

Wenk, SO ^{[1
]}

Kruip, J ^{[1
]}

机构：

[1] Ruhr Univ Bochum, Fac Biol, D-44780 Bochum, Germany

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2000年 / 737卷 / 1-2期

关键词：

Synechocystis; purification; photosystem I; proteins; membrane proteins;

D O I：

10.1016/S0378-4347(99)00525-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

New porous materials have been tested for their potential to speed up purification of membrane proteins. As an example the purification of photosystem I, a light-driven electron pump from the cyanobacterium Synechocystis PCC6803, was optimized. The combination of two HPLC steps (an anion-exchange chromatography followed by a hydrophobic interaction chromatography) yields homogeneous monomeric or trimeric photosystem I as determined by gel filtration and gel electrophoresis. In comparison to traditional purification schemes our method is at least three-times faster and allows for easy scale-up. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：131 / 142

页数：12

共 15 条

[1] FLOW-THROUGH PARTICLES FOR THE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF BIOMOLECULES - PERFUSION CHROMATOGRAPHY [J].