3D model of amphioxus steroid receptor complexed with estradiol

被引：13

作者：

Baker, Michael E. ^{[1
]}

Chang, David J. ^{[2
]}

机构：

[1] Univ Calif San Diego, Dept Med, La Jolla, CA 92093 USA

[2] Univ Calif San Diego, Dept Biol, La Jolla, CA 92093 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 386卷 / 03期

关键词：

Amphioxus steroid receptor; Steroid receptor evolution; Estrogen signaling; ESTROGEN-RECEPTOR; LIGAND-BINDING; NUCLEAR RECEPTOR; MINERALOCORTICOID RECEPTOR; PETROMYZON-MARINUS; CRYSTAL-STRUCTURE; SEX STEROIDS; EVOLUTION; SPECIFICITY; MECHANISMS;

D O I：

10.1016/j.bbrc.2009.06.079

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

The origins of signaling by vertebrate steroids are not fully understood. An important advance was the report that an estrogen-binding steroid receptor [SR] is present in amphioxus, a basal chordate with a similar body plan as vertebrates. To investigate the evolution of estrogen-binding to steroid receptors, we constructed a 3D model of amphioxus SR complexed with estradiol. This 3D model indicates that although the SR is activated by estradiol, some interactions between estradiol and human ER(x are not conserved in the SR, which can explain the low affinity of estradiol for the SR. These differences between the SR and ER alpha in the steroid-binding domain are sufficient to suggest that another steroid is the physiological regulator of the SR. The 3D model predicts that mutation of Glu-346 to Gln will increase the affinity of testosterone for amphioxus SR and elucidate the evolution of steroid-binding to nuclear receptors. (C) 2009 Elsevier Inc. All rights reserved,

引用

页码：516 / 520

页数：5

共 34 条

[1]

Co-evolution of steroidogenic and steroid-inactivating enzymes and adrenal and sex steroid receptors [J].

Baker, ME .

MOLECULAR AND CELLULAR ENDOCRINOLOGY, 2004, 215 (1-2) :55-62

[2]

Evolution of adrenal and sex steroid action in vertebrates: a ligand-based mechanism for complexity [J].

Baker, ME .

BIOESSAYS, 2003, 25 (04) :396-400

[3]

Recent insights into the origins of adrenal and sex steroid receptors [J].

Baker, ME .

JOURNAL OF MOLECULAR ENDOCRINOLOGY, 2002, 28 (03) :149-152

[4]

Adrenal and sex steroid receptor evolution: environmental implications [J].

Baker, ME .

JOURNAL OF MOLECULAR ENDOCRINOLOGY, 2001, 26 (02) :119-125

[5]

Steroid receptor phylogeny and vertebrate origins [J].

Baker, ME .

MOLECULAR AND CELLULAR ENDOCRINOLOGY, 1997, 135 (02) :101-107

[6]

Amphioxus, a primitive chordate, is on steroids: Evidence for sex steroids and steroidogenic enzymes [J].

Baker, Michael E. .

ENDOCRINOLOGY, 2007, 148 (08) :3551-3553

[7]

Evolutionary genomics of nuclear receptors: From twenty-five ancestral genes to derived endocrine systems [J].

Bertrand, W ;

Brunet, FG ;

Escriva, H ;

Parmentier, G ;

Laudet, V ;

Robinson-Rechavi, M .

MOLECULAR BIOLOGY AND EVOLUTION, 2004, 21 (10) :1923-1937

[8]

Crystal structure of the glucocorticoid receptor ligand binding domain reveals a novel mode of receptor dimerization and coactivator recognition [J].

Bledsoe, RK ;

Montana, VG ;

Stanley, TB ;

Delves, CJ ;

Apolito, CJ ;

McKee, DD ;

Consler, TG ;

Parks, DJ ;

Stewart, EL ;

Willson, TM ;

Lambert, MH ;

Moore, JT ;

Pearce, KH ;

Xu, HE .

CELL, 2002, 110 (01) :93-105

[9]

Evolution of a New Function by Degenerative Mutation in Cephalochordate Steroid Receptors [J].

Bridgham, Jamie T. ;

Brown, Justine E. ;

Rodriguez-Mari, Adriana ;

Catchen, Julian M. ;

Thornton, Joseph W. .

PLOS GENETICS, 2008, 4 (09)

[10]

Molecular basis of agonism and antagonism in the oestrogen receptor [J].

Brzozowski, AM ;

Pike, ACW ;

Dauter, Z ;

Hubbard, RE ;

Bonn, T ;

Engstrom, O ;

Ohman, L ;

Greene, GL ;

Gustafsson, JA ;

Carlquist, M .

NATURE, 1997, 389 (6652) :753-758

← 1 2 3 4 →