Uptake by human glioma cell lines and biological effects of a peptide-nucleic acids targeting miR-221

被引：55

作者：

Brognara, Eleonora ^{[1
]}

Fabbri, Enrica ^{[1
]}

Bazzoli, Elena ^{[2
,3
]}

Montagner, Giulia ^{[1
]}

Ghimenton, Claudio ^{[4
]}

Eccher, Albino ^{[4
]}

Cantu, Cinzia ^{[5
]}

Manicardi, Alex ^{[6
]}

Bianchi, Nicoletta ^{[1
]}

Finotti, Alessia ^{[1
]}

Breveglieri, Giulia ^{[1
]}

Borgatti, Monica ^{[1
]}

Corradini, Roberto

Bezzerri, Valentino ^{[4
,5
]}

Cabrini, Giulio ^{[4
,5
]}

Gambari, Roberto ^{[1
,6
]}

机构：

[1] Univ Ferrara, Dept Life Sci & Biotechnol, I-44100 Ferrara, Italy

[2] FBF, IRCCS Ctr San Giovanni di Dio, Genet Unit, Brescia, Italy

[3] Univ Verona, Dept Neurol Neuropsychol Morphol & Movement Sci, I-37100 Verona, Italy

[4] Univ Hosp Verona, Dept Pathol & Diagnost, Verona, Italy

[5] Univ Hosp, Lab Mol Pathol, Verona, Italy

[6] Univ Parma, Dept Chem, I-43100 Parma, Italy

来源：

JOURNAL OF NEURO-ONCOLOGY | 2014年 / 118卷 / 01期

关键词：

Peptide nucleic acids; Glioma; MicroRNAs; MiR-221; P27(Kip1); MiRNA targeting; Delivery; TRANSCRIPTION FACTOR; GENE-EXPRESSION; P27(KIP1) EXPRESSION; DOWN-REGULATION; DRUG-DELIVERY; MESSENGER-RNA; IN-VIVO; PNA; GROWTH; DNA;

D O I：

10.1007/s11060-014-1405-6

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

MicroRNAs are a family of small noncoding RNAs regulating gene expression by sequence-selective mRNA targeting, leading to a translational repression or mRNA degradation. The oncomiR miR-221 is highly expressed in human gliomas, as confirmed in this study in samples of low and high grade gliomas, as well in the cell lines U251, U373 and T98G. In order to alter the biological functions of miR-221, a peptide nucleic acid targeting miR-221 (R8-PNA-a221) was produced, bearing a oligoarginine peptide (R8) to facilitate uptake by glioma cells. The effects of R8-PNA-a221 were analyzed in U251, U373 and T98G glioma cells and found to strongly inhibit miR-221. In addition, the effects of R8-PNA-a221 on p27(Kip1) (a target of miR-221) were analyzed in U251 and T98G cells by RT-qPCR and by Western blotting. No change of p27(Kip1) mRNA content occurs in U251 cells in the presence of PNA-a221 (lacking the R8 peptide), whereas significant increase of p27(Kip1) mRNA was observed with the R8-PNA-a221. These data were confirmed by Western blot assay. A clear increment of p27(Kip1) protein expression in the samples treated with R8-PNA-a221 was detected. In addition, R8-PNA-a221 was found able to increase TIMP3 expression (another target of miR-221) in T98G cells. These results suggest that PNAs against oncomiRNA miR-221 might be proposed for experimental treatment of human gliomas.

引用

页码：19 / 28

页数：10

共 49 条

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