Evolution of shape complementarity and catalytic efficiency from a primordial antibody template

被引：105

作者：

Xu, JA

Deng, QL

Chen, JG

Houk, KN

Bartek, J

Hilvert, D

Wilson, IA

机构：

[1] Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA

[2] Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA

[3] Univ Calif Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90095 USA

[4] Swiss Fed Inst Technol, ETH Zurich, Organ Chem Lab, CH-8092 Zurich, Switzerland

来源：

SCIENCE | 1999年 / 286卷 / 5448期

关键词：

D O I：

10.1126/science.286.5448.2345

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The crystal structure of an efficient Diels-Alder antibody catalyst at 1.9 angstrom resolution reveals almost perfect shape complementarity with its transition state analog. Comparison with highly related progesterone and Diels-Alderase antibodies that arose from the same primordial germ Line template shows the relatively subtle mutational steps that were able to evolve both structural complementarity and catalytic efficiency.

引用

页码：2345 / 2348

页数：4

共 52 条

[1] NEW METHOD FOR PREDICTING BINDING-AFFINITY IN COMPUTER-AIDED DRUG DESIGN [J].