Three-dimensional bioprinting of rat embryonic neural cells

被引：125

作者：

Lee, Wonhye ^{[1
,2
]}

Pinckney, Jason ^{[1
]}

Lee, Vivian ^{[2
]}

Lee, Jong-Hwan ^{[1
]}

Fischer, Krisztina ^{[1
]}

Polio, Samuel ^{[1
]}

Park, Je-Kyun ^{[2
]}

Yoo, Seung-Schik ^{[1
,2
]}

机构：

[1] Harvard Univ, Sch Med, Brigham & Womens Hosp, Dept Radiol, Boston, MA 02115 USA

[2] Korea Adv Inst Sci & Technol, Coll Life Sci & Bioengn, Dept Bio & Brain Engn, Taejon 305701, South Korea

来源：

NEUROREPORT | 2009年 / 20卷 / 08期

关键词：

astrocytes; cell printing; collagen hydrogel; freeform fabrication; neurons; tissue engineering; EXTRACELLULAR-MATRIX; NEURONS; MORPHOLOGY; CULTURE; GROWTH; GELS;

D O I：

10.1097/WNR.0b013e32832b8be4

中图分类号：

Q189 [神经科学];

学科分类号：

071006 [神经生物学];

摘要：

We present a direct cell printing technique to pattern neural cells in a three-dimensional (3D) multilayered collagen gel. A layer of collagen precursor was printed to provide a scaffold for the cells, and the rat embryonic neurons and astrocytes were subsequently printed on the layer. A solution of sodium bicarbonate was applied to the cell containing collagen layer as nebulized aerosols, which allowed the gelation of the collagen. This process was repeated layer-by-layer to construct the 3D cell-hydrogel composites. Upon characterizing the relationship between printing resolutions and the growth of printed neural cells, single/multiple layers of neural cell-hydrogel composites were constructed and cultured. The on-demand capability to print neural cells in a multilayered hydrogel scaffold offers flexibility in generating artificial 3D neural tissue composites. NeuroReport 20:798-803 (C) 2009 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.

引用

页码：798 / 803

页数：6

共 21 条

[1]

Cell and organ printing 2: Fusion of cell aggregates in three-dimensional gels [J].